Rabbit Immunoglobulin E,IgE ELISA Kit

Rabbit Immunoglobulin E,IgE ELISA Kit

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中文名稱：

兔子免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06948Rb
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
促銷：
其他：

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產(chǎn)品描述：

兔子免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E06948Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IgE含量。IgE即免疫球蛋白E，是介導(dǎo)Ⅰ型超敏反應(yīng)的抗體，在過敏反應(yīng)、寄生蟲感染等過程中發(fā)揮重要作用。研究機(jī)制上，其可與肥大細(xì)胞和嗜堿性粒細(xì)胞表面受體結(jié)合，當(dāng)變應(yīng)原再次入侵，結(jié)合IgE后促使細(xì)胞釋放炎癥介質(zhì)，引發(fā)過敏癥狀。試劑盒檢測(cè)范圍為7.8 pg/mL-2000 pg/mL，適用于科研場(chǎng)景中兔模型過敏性炎癥機(jī)制探索、寄生蟲感染免疫動(dòng)態(tài)監(jiān)測(cè)、疫苗或藥物干預(yù)實(shí)驗(yàn)的療效評(píng)估等研究，尤其適合需要高靈敏度檢測(cè)微量樣本的應(yīng)用需求。試劑盒兼容血清與血漿樣本，為體外實(shí)驗(yàn)提供便捷的檢測(cè)方案，滿足基礎(chǔ)研究中對(duì)免疫指標(biāo)定量分析的要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgE
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測(cè)范圍：

7.8 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	91
	Range %	84-96
1:2000	Average %	89
	Range %	82-96
1:4000	Average %	93
	Range %	87-100
1:8000	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	88	85-91

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.218	0.220	0.219
500	0.353	0.354	0.354
125	0.622	0.634	0.628
31.25	1.523	1.436	1.480
7.8	2.389	2.226	2.308
0	3.127	3.244	3.186

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.