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Rabbit Coagulation factor VII(F7) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

兔凝血因子VII(F7)酶聯(lián)免疫試劑盒（CSB-EL007930RB）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F7含量。試劑盒檢測范圍為3.9 ng/mL-250 ng/mL，適用于科研領(lǐng)域?qū)δ獧C制、出血性疾病模型或藥物干預(yù)效果的研究；支持研究者高效完成心血管疾病模型、肝臟功能評估或抗凝藥物開發(fā)等實驗中的F7水平監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
縮寫：

F7
Uniprot No.：

P98139
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.9 ng/mL-250 ng/mL
靈敏度：

1 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-90
1:40	Average %	104
	Range %	100-107
1:80	Average %	87
	Range %	83-90
1:160	Average %	102
	Range %	98-106

回收率：

The recovery of rabbit F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	110	107-113

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能參考文獻：
1. Results suggest that inhibition of FVIIa with small-molecule active-site inhibitors represents a promising antithrombotic approach. PMID: 20589312
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
組織特異性：

Plasma.
數(shù)據(jù)庫鏈接：

KEGG: ocu:100009399

STRING: 9986.ENSOCUP00000024648

UniGene: Ocu.2617