Pig interleukin 8,IL-8 ELISA Kit

Pig interleukin 8,IL-8 ELISA Kit

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中文名稱：

豬白介素8(IL-8/CXCL8)酶聯免疫試劑盒
貨號：

CSB-E06787p
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬白介素8(IL-8/CXCL8)酶聯免疫試劑盒（CSB-E06787p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CXCL8含量。CXCL8，即CXC趨化因子配體8，也稱為白細胞介素-8，是一種重要的趨化因子，主要由免疫細胞和腫瘤細胞分泌。它通過激活中性粒細胞和其他免疫細胞，參與炎癥反應和腫瘤的發生發展。CXCL8的研究機制涉及與受體CXCR1和CXCR2的結合，以及下游信號通路的激活，如MAPK和PI3K/Akt通路。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，靈敏度為31.25 pg/mL。適用于體外細胞因子分泌分析、感染或炎癥模型中免疫應答評估、疫苗研發過程中的免疫效果監測等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P26894
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	90
	Range %	86-94
1:4	Average %	88
	Range %	84-93
1:8	Average %	92
	Range %	88-97

回收率：

The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	85	81-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.662	2.601	2.632	2.460
4000	2.215	2.161	2.188	2.016
2000	1.498	1.404	1.451	1.279
1000	0.871	0.819	0.845	0.673
500	0.510	0.507	0.509	0.337
250	0.380	0.359	0.370	0.198
125	0.271	0.261	0.266	0.094
0	0.174	0.170	0.172

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Taurine ameliorates deoxynivalenol-induced intestinal injury in piglets: Restoration of mitochondrial function linked to the PGC1α-NRF1/2 axis X Ji, H Ding, F Zhou, F Zhang, D Wu,Ecotoxicology and Environmental Safety,2025
Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages Jingxia Gao,Frontiers in immunology,2022
Porcine circovirus type 2 upregulates endothelial-derived IL-8 production in porcine iliac artery endothelial cells via the RIG-I/MDA-5/MAVS/JNK signaling pathway F Shi,BMC Veterinary Research,2020
Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1 Qingwei Meng.et al,J Anim Sci Biotechnol.,2018
The Effect of Divergence in Feed Efficiency on the Intestinal Microbiota and the Intestinal Immune Response in Both Unchallenged and Lipopolysaccharide Challenged Ileal and Colonic Explants. Vigors S. et al,PLoS One.,2016

平均分:

5.0分 - 1 個評價

樣品類型：細胞培養上清液

樣品信息：其他

稀釋比：沒有稀釋

產品評價：結構很好，已發文章

By 高老師

最新研究進展：

IL8，也稱為白細胞介素-8，是一種細胞因子蛋白質，參與炎癥和免疫反應。它主要由激活的單核細胞和巨噬細胞產生，在炎癥、感染和損傷等條件下被釋放。IL8作為趨化因子，可以引導白細胞移動到感染或受損組織，促進炎癥反應和免疫細胞的增殖和激活。最近的研究表明，IL8也參與許多其他生物學過程，如細胞增殖、生存、轉移和血管新生等。
功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能參考文獻：
1. This study evaluated the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-kappaB activation. PMID: 29521102
2. The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV PMID: 28347884
3. These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway. PMID: 26581603
4. Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. PMID: 25575706
5. Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine. PMID: 25147235
6. downregulated gene expression elite controllers (fraction of human population displaying resistance to HIV infection) PMID: 23751822
7. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
9. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
10. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
11. Classical swine fever virus NS2 protein promotes interleukin-8 expression. PMID: 21318239
12. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth PMID: 19232500
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
組織特異性：

Alveolar macrophages.
數據庫鏈接：

KEGG: ssc:396880

STRING: 9823.ENSSSCP00000026976

UniGene: Ssc.658

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.