Pig hepatocyte growth factor,HGF ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬肝細(xì)胞生長因子(HGF)酶聯(lián)免疫試劑盒（CSB-E06795p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HGF含量。HGF（肝細(xì)胞生長因子）是一種多功能細(xì)胞因子，在胚胎發(fā)育、組織再生與修復(fù)等生理過程中發(fā)揮關(guān)鍵作用。其異常表達(dá)與腫瘤生長、侵襲和轉(zhuǎn)移密切相關(guān)。研究機(jī)制主要是通過與受體c-Met結(jié)合，激活下游信號(hào)通路，影響細(xì)胞的增殖、存活等。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，可廣泛應(yīng)用于肝臟再生機(jī)制研究、疾病模型（如肝纖維化或損傷修復(fù)）中HGF動(dòng)態(tài)變化的檢測(cè)，以及藥物或生物制劑干預(yù)后HGF調(diào)控效應(yīng)的評(píng)估，為相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HGF
Uniprot No.：

F1SB93
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	104
	Range %	94-110
1:4	Average %	95
	Range %	89-99
1:8	Average %	94
	Range %	86-97

回收率：

The recovery of pig HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-95
EDTA plasma (n=4)	97	92-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.