Pig Renin ELISA Kit

說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

豬腎素(Renin)酶聯(lián)免疫試劑盒（CSB-E17914p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的REN含量。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于科研場(chǎng)景中與Renin相關(guān)的動(dòng)物模型研究，如高血壓機(jī)制探索、腎臟病理分析或藥物干預(yù)實(shí)驗(yàn)，為評(píng)估豬心血管及腎臟系統(tǒng)的生理病理變化提供可靠工具，滿足基礎(chǔ)研究中對(duì)目標(biāo)蛋白精準(zhǔn)定量的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

FLJ10761, HNFJ2, angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
縮寫(xiě)：

REN
種屬：

Sus scrofa (Pig)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	92
	Range %	85-102
1:4	Average %	93
	Range %	83-99
1:8	Average %	95
	Range %	90-110

回收率：

The recovery of pig renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-102
EDTA plasma (n=4)	94	86-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.