Dog Renin ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬腎素(Renin)酶聯(lián)免疫試劑盒（CSB-E08704c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的REN含量。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于實(shí)驗(yàn)動(dòng)物模型（如高血壓、腎功能損傷等）中腎素活性變化的動(dòng)態(tài)監(jiān)測(cè)，或用于藥物干預(yù)、病理生理機(jī)制研究中的分子水平分析，為心血管及代謝性疾病的基礎(chǔ)科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
縮寫：

REN
Uniprot No.：

Q6DYE7
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	105
	Range %	100-112
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	88	84-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
基因功能參考文獻(xiàn)：
1. With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID: 19930429
2. Characterization of canine renin with mutations in the substrate binding site. PMID: 17942478
3. Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID: 19073902
亞細(xì)胞定位：

Secreted. Membrane.
蛋白家族：

Peptidase A1 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:403838

STRING: 9615.ENSCAFP00000014146

UniGene: Cfa.3701