Pig Progonadoliberin-2(GNRH2) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬促性腺素釋放素原-2(GNRH2)酶聯免疫試劑盒（CSB-EL009636PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GNRH2含量。GNRH2即促性腺激素釋放激素2，它在生殖內分泌系統中扮演重要角色。研究認為它通過與特異性受體結合，調控垂體促性腺激素的合成與分泌，進而影響生殖功能。目前對其作用機制的深入研究，有望為生殖疾病的診治提供新方向。試劑盒檢測范圍為40 pg/mL-8000 pg/mL，廣泛應用于動物生殖生理機制研究，包括繁殖性能評估、生殖內分泌調控網絡解析以及激素相關疾病模型的分子機制探索，也可為畜牧業中繁殖管理策略的優化提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II
縮寫：

GNRH2
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

40 pg/mL-8000 pg/mL
靈敏度：

20 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	90-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	88-103

回收率：

The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	89-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.