Pig Interleukin 18,IL-18 ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬白介素18(IL-18)酶聯免疫試劑盒（CSB-E06781p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL18含量。IL18 即白細胞介素 18，是一種多功能促炎細胞因子，在先天免疫和獲得性免疫中均發揮重要作用。其研究機制主要圍繞它能誘導 T 細胞和自然殺傷細胞產生γ-干擾素，增強自然殺傷細胞的細胞毒活性，在炎癥、免疫相關疾病研究中備受關注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研場景中動態監測實驗動物模型（如細菌/病毒感染、免疫調節研究）的免疫應答水平；為獸醫學、比較免疫學及動物疾病模型研究提供可靠工具，助力科研人員解析IL - 18在豬免疫系統中的分子機制與調控網絡。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
縮寫：

IL18
Uniprot No.：

O19073
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	91-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
基因功能參考文獻：
1. Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
2. IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
3. endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
4. Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
亞細胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

KEGG: ssc:397057

STRING: 9823.ENSSSCP00000015962

UniGene: Ssc.20