Pig Interferon β,IFN-β/IFNB ELISA Kit

產品詳情

文獻引用 (12) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬β干擾素(IFN-β/IFNB)酶聯免疫試劑盒（CSB-E09890p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干擾素，它具有抗病毒、免疫調節等作用。在研究機制上，它能與細胞表面受體結合，激活相關信號通路，誘導抗病毒蛋白產生，從而抑制病毒復制。目前在治療多發性硬化癥等疾病方面有重要研究價值。試劑盒檢測范圍為50 pg/mL-2000 pg/mL，該產品適用于體外科研場景中豬IFN-β的動態水平監測，如病毒感染后免疫應答研究、炎癥模型評估或生物制劑開發過程中的效價分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IFN-β/IFNB
Uniprot No.：

Q29412
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

50 pg/mL-2000 pg/mL
靈敏度：

25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-86
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	95
	Range %	90-99

回收率：

The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	84	80-88