Pig Alpha-fetoprotein(AFP) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬甲胎蛋白(AFP)酶聯免疫試劑盒（CSB-EL001421PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的AFP含量。AFP即甲胎蛋白，是一種糖蛋白。它在胎兒早期由肝臟和卵黃囊合成。在肝癌等多種惡性腫瘤中會異常升高，臨床上常用作腫瘤標志物輔助診斷。研究機制集中于其表達調控及與腫瘤細胞增殖、轉移等過程的關聯，以探尋新的治療靶點。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于豬模型研究中肝臟疾病機制探索、腫瘤發生發展監測、生殖生理學研究等科研領域，滿足體外實驗中對AFP蛋白表達水平動態變化的精準追蹤需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
縮寫：

AFP
Uniprot No.：

Q8MJ76
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-102
1:2	Average %	92
	Range %	87-96
1:4	Average %	88
	Range %	82-94
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	91	86-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
亞細胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
組織特異性：

Plasma. Synthesized by the fetal liver and yolk sac.
數據庫鏈接：

KEGG: ssc:397586

STRING: 9823.ENSSSCP00000009550

UniGene: Ssc.7131