Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

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中文名稱：

小鼠末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08710m
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)酶聯(lián)免疫試劑盒（CSB-E08710m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TCC C5b9含量。小鼠末端補(bǔ)體復(fù)合物C5b-9（TCC C5b-9）是補(bǔ)體系統(tǒng)激活終末階段的產(chǎn)物，通過(guò)介導(dǎo)細(xì)胞膜穿孔參與免疫防御和炎癥反應(yīng)調(diào)控。其在膜性腎病等免疫相關(guān)疾病中發(fā)揮重要作用，研究其機(jī)制有助于揭示疾病發(fā)病機(jī)制，為疾病治療提供新靶點(diǎn)。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，該產(chǎn)品適用于科研場(chǎng)景中炎癥性疾病模型評(píng)估、補(bǔ)體通路功能研究或藥物干預(yù)效果分析，為探索免疫調(diào)節(jié)機(jī)制提供可靠工具，所有應(yīng)用僅限于實(shí)驗(yàn)室研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

TCC C5b9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.684	2.810	2.747	2.596
25	2.054	2.212	2.133	1.982
12.5	1.426	1.446	1.436	1.285
6.25	0.854	0.867	0.861	0.710
3.12	0.622	0.585	0.604	0.453
1.56	0.337	0.350	0.344	0.193
0.78	0.258	0.264	0.261	0.110
0	0.148	0.154	0.151

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.