Mouse telomerase,TE ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠端粒酶(TE)酶聯(lián)免疫試劑盒（CSB-E08022m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TE含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒產(chǎn)品廣泛應(yīng)用于科研領(lǐng)域，包括但不限于端粒酶活性相關(guān)的基礎(chǔ)機(jī)制研究、抗衰老藥物篩選、腫瘤細(xì)胞增殖模型構(gòu)建以及再生醫(yī)學(xué)中的干細(xì)胞功能評估等實(shí)驗(yàn)場景，為探索TE在疾病發(fā)生發(fā)展中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

TE
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	82-91
1:10	Average %	104
	Range %	100-108
1:20	Average %	99
	Range %	96-105
1:40	Average %	91
	Range %	87-99

回收率：

The recovery of mouse TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	88	83-91

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.