Human telomerase,TE ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 評(píng)價(jià) (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人端粒酶(TE)酶聯(lián)免疫試劑盒（CSB-E08021h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的TE含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，科研人員可通過(guò)分析疾病模型動(dòng)物或體外培養(yǎng)細(xì)胞中的端粒酶表達(dá)動(dòng)態(tài)，探索腫瘤發(fā)生機(jī)制、評(píng)估抗衰老藥物對(duì)端粒酶活性的調(diào)控作用，或利用尿液樣本開(kāi)展無(wú)創(chuàng)性研究。其寬泛的樣本兼容性為組織水平研究、細(xì)胞實(shí)驗(yàn)數(shù)據(jù)驗(yàn)證及生物標(biāo)志物篩選提供了可靠工具，適用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及分子生物學(xué)領(lǐng)域的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

TE
種屬：

Homo sapiens (Human)
樣本類型：

serum, cell culture supernates, urine, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	99
	Range %	91-105
1:4	Average %	94
	Range %	92-98
1:8	Average %	89
	Range %	86-100

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.