Mouse pyridinoline (PYD) ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠膠原吡啶交聯(PYD)酶聯免疫試劑盒（CSB-E15010m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PYD含量。小鼠膠原吡啶交聯（PYD）是一種通過吡啶交聯劑對膠原蛋白進行交聯的技術，旨在改善膠原蛋白的物理和生物性能。該技術通過交聯反應增加膠原蛋白的穩定性和機械強度，廣泛應用于組織工程和再生醫學領域，以促進組織修復和再生。試劑盒檢測范圍為50 ng/mL-800 ng/mL，適用于骨代謝機制研究、骨質疏松動物模型評估、抗骨吸收藥物開發等科研領域，為骨代謝相關基礎研究提供高效可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PYD
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

50 ng/mL-800 ng/mL
靈敏度：

50 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	100
	Range %	92-106
1:4	Average %	85
	Range %	80-90
1:8	Average %	91
	Range %	83-99

回收率：

The recovery of mouse PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-97
EDTA plasma (n=4)	93	91-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.