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中文名稱：人膠原吡啶交聯(PYD)酶聯免疫試劑盒
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貨號：CSB-E09702h
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人膠原吡啶交聯(PYD)酶聯免疫試劑盒（CSB-E09702h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的PYD含量。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于骨代謝機制研究、抗骨質疏松藥物篩選、關節炎動物模型評估等科研領域。特別針對尿液樣本的兼容性設計，為無創性骨代謝動態監測提供了便捷方案，可滿足體外培養體系檢測、疾病模型縱向觀察等實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：N/A
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縮寫：PYD
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, urine
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檢測范圍：12.5 ng/mL-800 ng/mL
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靈敏度：3.12 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 88-100 1:2 Average % 92 Range % 86-99 1:4 Average % 87 Range % 80-94 1:8 Average % 97 Range % 92-102 -
回收率：
The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 85-99 EDTA plasma (n=4) 98 92-104 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 800 2.501 2.450 2.476 2.343 400 1.934 2.009 1.972 1.839 200 1.295 1.301 1.298 1.165 100 0.718 0.775 0.747 0.614 50 0.483 0.495 0.489 0.356 25 0.331 0.351 0.341 0.208 12.5 0.226 0.235 0.231 0.098 0 0.136 0.129 0.133 -
數據處理：
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貨期：3-5 working days
引用文獻
- The effect of n-3 fatty acids on bone biomarkers in Iranian postmenopausal osteoporotic women: a randomized clinical trial /,Journal of the american aging association,2010
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