Mouse plasma kallikrein(KLKB1) ELISA Kit

Mouse plasma kallikrein(KLKB1) ELISA Kit

產品詳情

引用文獻

產品評價

靶點詳情

產品詳情

引用文獻

產品評價

相關產品

靶點詳情

Most popular with customers

產品中心

信號通路

技術信息

聯系信息

文獻引用 (1) 說明書下載

中文名稱：

小鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒
貨號：

CSB-E16637m
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品描述：

小鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒（CSB-E16637m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KLKB1含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，支持基礎科研中KLKB1相關機制研究，例如炎癥模型中的激肽系統動態分析、藥物干預對凝血 - 纖溶平衡的影響評估，或代謝性疾病動物模型中酶活性變化的探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
縮寫：

KLKB1
Uniprot No.：

P26262
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	96-102
1:2000	Average %	103
	Range %	100-106
1:4000	Average %	89
	Range %	84-94
1:8000	Average %	96
	Range %	94-98

回收率：

The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	103	100-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.459	2.555	2.507	2.388
100	1.877	1.966	1.922	1.803
50	1.355	1.332	1.344	1.225
25	0.789	0.768	0.779	0.660
12.5	0.546	0.588	0.567	0.448
6.25	0.323	0.345	0.334	0.215
3.12	0.204	0.221	0.213	0.094
0	0.118	0.119	0.119

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Inhibition of plasma kallikrein mitigates experimental hypertension-enhanced cerebral hematoma expansion J Wang,Brain Research Bulletin,2021

功能：

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
基因功能參考文獻：
1. Plasma kallikrein mediates brain hemorrhage and edema caused by tissue plasminogen activator therapy in mice after stroke. PMID: 28130211
2. we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
3. Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. PMID: 22398951
4. PKK or fXII deficiency reduced thrombus formation in both arterial and venous thrombosis models, without an apparent effect on hemostasis. PMID: 21821705
5. the prekallikrein gene (Klkb1) is expressed highly in the mammary gland during stromal remodeling periods including puberty and postlactational involution. PMID: 19297327
顯示更多

收起更多
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family, Plasma kallikrein subfamily
數據庫鏈接：

KEGG: mmu:16621

STRING: 10090.ENSMUSP00000112174

UniGene: Mm.482691

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.