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Mouse parathyroid hormone (PTH) ELISA kit

文獻引用 (4) 說明書下載

產品詳情

產品描述：

小鼠甲狀旁腺激素(PTH)酶聯免疫試劑盒（CSB-E08690m）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織勻漿樣本中的PTH含量。PTH（甲狀旁腺激素）是一種調節血鈣水平的典型內分泌激素，對維持鈣磷代謝和骨骼健康至關重要。其通過結合骨細胞和腎臟細胞中的受體PTH1R，激活下游信號通路，調節鈣磷代謝。近年來，PTH及其相關肽（PTHrPs）在骨質疏松癥治療等領域展現出顯著療效，成為研究熱點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產品適用于基礎研究中的鈣代謝調控機制探索、甲狀旁腺功能相關疾病模型建立、藥物干預效果評估等方向，特別針對骨代謝研究、腎性骨營養不良模型分析及內分泌系統相關實驗提供可靠檢測工具，為科研人員解析PTH在生理病理過程中的作用機制提供標準化解決方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PTH
種屬：

Mus musculus (Mouse)
樣本類型：

serum, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-99
1:2	Average %	100
	Range %	96-104
1:4	Average %	90
	Range %	84-94
1:8	Average %	96
	Range %	89-100

回收率：

The recovery of mouse PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-95
EDTA plasma (n=4)	104	97-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.