Rat Parathyroid hormone,PTH ELISA Kit
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中文名稱：大鼠甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒
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貨號：CSB-E07866r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒（CSB-E07866r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PTH含量。PTH即甲狀旁腺激素，是甲狀旁腺主細(xì)胞分泌的肽類激素。它主要作用于骨和腎臟，調(diào)節(jié)鈣磷代謝，維持血鈣平衡。研究機(jī)制上，其可通過與受體結(jié)合激活信號通路，影響骨重建和腎小管對鈣磷的重吸收等。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品廣泛應(yīng)用于內(nèi)分泌機(jī)制研究、骨代謝動(dòng)物模型評價(jià)、藥物干預(yù)效果驗(yàn)證等領(lǐng)域，尤其適用于探究甲狀旁腺相關(guān)疾病模型中的激素動(dòng)態(tài)變化，或結(jié)合其他鈣調(diào)節(jié)因子進(jìn)行系統(tǒng)性機(jī)制解析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Pth ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：6.25 pg/mL-400 pg/mL
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靈敏度：1.56 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 97 Range % 91-103 1:10 Average % 99 Range % 95-107 1:20 Average % 95 Range % 90-99 1:40 Average % 87 Range % 81-92 -
回收率：
The recovery of rat PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-97 EDTA plasma (n=4) 106 102-109 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.613 2.511 2.562 2.408 200 2.054 1.953 2.004 1.850 100 1.276 1.287 1.282 1.128 50 0.727 0.738 0.733 0.579 25 0.457 0.468 0.463 0.309 12.5 0.361 0.350 0.356 0.202 6.25 0.275 0.269 0.272 0.118 0 0.154 0.153 0.154 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- METTL3 obstructs vascular smooth muscle cells osteogenic reprogramming by methylating Runx2 in chronic kidney disease M Cheng, J Jin, D Zhang, M Xiao, H Zhao,Communications Biology,2025
- Bone Developmental Toxicology Studies of Cadmium Chloride in Juvenile Sprague Dawley Rats S Wang,/,2024
- Research article Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and trabecular bone loss Azza M. El-Wakf,Journal of Innovations in Pharmaceutical and Biological Sciences,2019
- Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia Danijela Radojkovic.et al,Endocrine,2018
- Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats Turkyah J. Al-Bogami.et al,International Journal of Life Sciences Research,2016
產(chǎn)品評價(jià)
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靶點(diǎn)詳情
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功能：PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
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基因功能參考文獻(xiàn)：
- PTHrP and PTH mediate wasting through a common mechanism involving PTHR. PMID: 26669699
- our data demonstrate that PTH activation of nuclear receptor-associated protein 1 is essential for the effect of PTH to increase FGF23 levels. PMID: 24940803
- data support a link between PTH regulating HDAC4 phosphorylation by PKA, trafficking, partial degradation, and the control of MMP-13 transcription through association with Runx2 PMID: 24904057
- Mg reduces PTH secretion mainly when a moderate low calcium concentration is present; Mg also modulates parathyroid glands function through upregulation of the key cellular receptors CaR, VDR and FGF23/Klotho system. PMID: 24103811
- The persistence of the phosphaturic response to PTH occurs through posttranscriptional mechanisms. PMID: 23344572
- The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity. PMID: 21209610
- Data suggest that a high concentration of PTH(1-34)-induced cytotoxicity and apoptosis in PC12 cells was associated with upregulation of ERK and p38 through a mitochondria-mediated apoptotic pathway. PMID: 20971569
- Study indicates an influence of sex on PTH molecular form secretion or metabolism. PMID: 20627105
- Modulation of integrin expression in osteoblastic cells by PTH is likely to be indirect, and phenotypic expression of integrin subtypes is part of the cascade of events that lead to PTH (1-34) mediated osteoblast differentiation. PMID: 11746505
- New signaling pathway for parathyroid hormone and cyclic AMP action on extracellular-regulated kinase and cell proliferation in bone cells PMID: 11956184
- alterations in PI3K activity in response to parathyroid hormone with aging PMID: 12581861
- PTH or PTHrP can exert anabolic effects in the skeleton. PMID: 12631114
- that periosteal and endosteal osteoblastic cell populations exhibit metabolic differences in their extracellular matrix responses to PTH PMID: 14514685
- the parathyroid hormone-Smad3 axis may have a role in the bone anabolic action of PTH PMID: 14517210
- PTH and mechanical stimulation on trabecular bone formation rate suggest a potential benefit for combined PTH administration and exercise in the treatment of osteoporosis. PMID: 14672346
- Rho and Rho kinase are an important component of PTH signaling in osteoblastic cells PMID: 15476589
- Combinations of radioprotectors may achieve their beneficial additive effects in the growth plate by decreasing the usual early drop in parathyroid hormone-related protein and parathyroid hormone receptor 1 after irradiation PMID: 16494524
- Calcium, phosphate & uremia determine parathyroid hormone mRNA stability through binding of protective factors adenosine-uridine rich binding factor 1 & Up-stream of N-ras & recruitment of degradation complex that cleaves parathyroid hormone mRNA[review] PMID: 17565271
- FGF23 suppressed both parathyroid hormone (PTH) secretion and PTH gene expression PMID: 17992255
- IL-18 is regulated by parathyroid hormone and is required for its bone anabolic actions PMID: 18165223
- These data indicate a novel role for KSRP in parathyroid hormone gene expression. PMID: 18583400
- Phosphate overload and normal parathyroid hormone infusion induced phenotypic changes in vascular smooth muscle cells, as evidenced by upregulated aortic expression of Runx2. PMID: 19075196
- Regulation of PTH mRNA stability by the calcimimetic R568 and the phosphorus binder lanthanum carbonate in chronic kidney disease. PMID: 19129257
- without altering the gross chromatin structure, PTH stimulates acetylation of histones H3 and H4 via recruitment of p300 to Runx2 bound to the MMP-13 promoter, resulting in gene activation PMID: 19423655
- estrogens regulate PTH indirectly, possibly through FGF23 PMID: 19628670
- Parathyroid hormone (PTH) suppresses rat PTH/PTH-related protein receptor gene promoter PMID: 11554727
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亞細(xì)胞定位：Secreted.
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蛋白家族：Parathyroid hormone family
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組織特異性：Hypothalamus and parathyroid gland.
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