Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

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文獻引用 (15) 評價 (1) 說明書下載

中文名稱：

小鼠基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒
貨號：

CSB-E07417m
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒（CSB-E07417m）檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP-1含量。MMP-1，也稱為間質膠原酶和成纖維細胞膠原酶，是基質金屬蛋白酶家族的一員。它參與細胞外基質的降解，在正常生理過程和疾病過程中發揮作用。MMP-1通過降解膠原蛋白和明膠等成分，影響細胞微環境，與腫瘤侵襲、轉移和骨關節炎等疾病相關。研究MMP-1有助于開發針對相關疾病的治療策略。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于體外實驗研究，可支持細胞功能探索、疾病模型建立及藥物作用機制分析等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
縮寫：

MMP-1
Uniprot No.：

Q9EPL5
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
Range %	86-95
1:40	Average %	100
Range %	97-103
1:80	Average %	91
Range %	87-95
1:160	Average %	97
Range %	93-100

回收率：

The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.482	2.510	2.496	2.317
1500	1.855	1.786	1.821	1.642
750	1.246	1.232	1.239	1.060
375	0.748	0.774	0.761	0.582
187.5	0.495	0.456	0.476	0.297
93.75	0.322	0.339	0.331	0.152
46.88	0.253	0.242	0.248	0.069
0	0.180	0.178	0.179

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Protective effects of Colla Corii Asini Collagen Peptides on D-galactose injection combined with UVB irradiation-induced aging in mice Q Luo, S Zhang, Z Sun, Z Wang, Q Yue, X Sun, L Tian,PloS one,2025
Hybrid complex of tyrosinase and carbon-based materials for tissue engineering applications ???,????? ???,2024
In Vitro Biological Activities and Relative Chemicals in Different Extracts of Licorice and Fermenting Licorice Z Geng,Asian Journal of Beauty & Cosmetology,/
Single-Walled Carbon Nanotube-Guided Topical Skin Delivery of Tyrosinase to Prevent Photoinduced Damage J Ko,ACS nano,2023
Neuropeptide Y, a potential marker for lupus, promotes lupus development DC Wang,International immunopharmacology,2023
MitoQ combats tumor cell progression in Ehrlich ascites carcinoma mice: A crosstalk between mitochondrial oxidative status, mitophagy, and NF-κB signaling MA Oraby,Life sciences,2023
Intentional endometrial injury enhances angiogenesis through increased production and activation of MMP-9 by TNF-α and MMP-3 in a mouse model YA Tu,Molecular Human Reproduction,2021
Fermentation of Blackberry with L. plantarum JBMI F5 Enhance the Protection Effect on UVB-Mediated Photoaging in Human Foreskin Fibroblast and Hairless Mice through Regulation of MAPK/NF-κB Signaling Kim H R, et al,Nutrients,2019
Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling Jiang L, et al,Journal of Cellular Physiology,2019
Protective Effect of Super-Critical Carbon Dioxide Fluid Extract from Flowers and Buds of Chrysanthemum indicum Linnén Against Ultraviolet-Induced Photo-Aging in Mice Zhang X.et al,Rejuvenation research,2015
Photo-protective activity of pogostone against UV-induced skin premature aging in mice Wang XF.et al,Exp Gerontol.,2016
Garlic Supplementation Ameliorates UV-Induced Photoaging in Hairless Mice by Regulating Antioxidative Activity and MMPs Expression Kim HK,Molecules,2016
Changes in the expression and protein level of matrix metalloproteinases after exposure to waterpipe tobacco smoke Khabour OF. et al,Inhal Toxicol,2015
Protective Effect of Supercritical-Carbon Dioxide Fluid Extract from Flowers and Buds of Chrysanthemum indicum Linnén against UV-induced Photo-aging in Mice Zhang Xie et al,Rejuvenation Research,2015
Deletion of extracellular matrix metalloproteinase inducer/CD147 induces altered cardiac extracellular matrix remodeling in aging mice. Huet E. et al,J Physiol Pharmacol,2015

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平均分:

5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:10

產品評價：我用CSB-E07417m檢測小鼠血清MMP-1水平，其OD如圖所示，試劑盒樣本充足，使用說明簡潔明了，操作容易上手，有助于實驗進展，滿意。

By 劉老師

功能：

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Able to degrade synthetic peptides and type I and II fibrillar collagen.
基因功能參考文獻：
1. The difficulty to effectively maintain Mmp1a in the zymogen form may account for the tight control of Mmp1a expression and reduced expression in normal tissue as compared to inflammatory states or cancer. PMID: 24737602
2. Stromal Mmp1a drives in vivo tumorigenesis. PMID: 23708660
3. Matrix metalloproteinase Mmp-1a is not required for growth and fertility in mice and has a role in lung cancer progression by modulating inflammatory responses PMID: 23548910
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

KEGG: mmu:83995

STRING: 10090.ENSMUSP00000034492

UniGene: Mm.156952

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.