Mouse eosinophil cationic protein,ECP ELISA Kit

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠嗜酸性粒細(xì)胞陽離子蛋白(ECP)酶聯(lián)免疫試劑盒（CSB-E11799m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的ECP含量。ECP即嗜酸性粒細(xì)胞陽離子蛋白，在過敏等多種免疫相關(guān)疾病背景下發(fā)揮作用。研究機制方面，它具有細(xì)胞毒性，可破壞寄生蟲、腫瘤細(xì)胞等，還能趨化炎癥細(xì)胞，參與炎癥反應(yīng)的調(diào)控，在疾病發(fā)生發(fā)展中扮演重要角色，是潛在治療靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研場景中評估疾病模型（如哮喘、過敏或寄生蟲感染）的嗜酸性粒細(xì)胞激活程度，支持多種樣本類型的平行檢測，為研究 Th2 型免疫反應(yīng)、組織炎癥機制及藥物干預(yù)效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ECP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-106
1:4	Average %	93
	Range %	88-99
1:8	Average %	89
	Range %	83-95

回收率：

The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.