Mouse catestatin ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠兒茶酚抑素酶聯免疫試劑盒（CSB-E17357m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的catestatin含量。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于體外培養細胞模型中CST分泌水平的動態監測、疾病動物模型（如高血壓或心肌缺血模型）組織內源性CST表達分析，以及探索CST在代謝綜合征相關機制中的作用，為神經內分泌調控、心血管病理機制等基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

catestatin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
Range %	87-96
1:2	Average %	99
Range %	95-103
1:4	Average %	101
Range %	94-106
1:8	Average %	95
Range %	89-98

回收率：

The recovery of mouse catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-98
EDTA plasma (n=4)	99	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.