Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

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中文名稱：

小鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒
貨號：

CSB-E04495m
規(guī)格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠血管緊張素Ⅱ(ANG-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E04495m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ANG-Ⅱ含量。ANG-Ⅱ即血管緊張素Ⅱ，在人體血壓調(diào)節(jié)、體液平衡等生理過程中扮演關鍵角色。其過度激活與高血壓、心血管疾病等多種病癥相關。研究主要聚焦于其信號傳導通路、與受體相互作用機制，為研發(fā)針對性藥物以防治相關疾病提供理論依據(jù)。試劑盒檢測范圍為15.6 pg/ml-1000 pg/ml.，適用于心血管疾病動物模型研究、降壓藥物作用機制探索、內(nèi)分泌調(diào)控實驗等科研領域，為小鼠血清或血漿樣本中ANG - Ⅱ的動態(tài)監(jiān)測提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

angiotensin Ⅱ, angiotensin 2, ANG2
縮寫：

ANG-Ⅱ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/ml-1000 pg/ml.
靈敏度：

7.8 pg/ml.
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-94
1:2	Average %	91
	Range %	85-96
1:4	Average %	90
	Range %	84-98
1:8	Average %	93
	Range %	86-99

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

1000

0.161

0.166

0.164

500

0.185

0.192

0.189

250

0.228

0.237

0.233

125

0.334

0.324

0.329

62.5

0.568

0.576

0.572

31.25

0.902

0.891

0.897

15.6

1.208

1.217

1.212

2.176

2.223

2.199

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Human placental mesenchymal stem cells transplantation repairs the alveolar epithelial barrier to alleviate lipopolysaccharides-induced acute lung injury W Yu, Y Lv, R Xuan, P Han, H Xu, X Ma,Biochemical Pharmacology,2024
ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway F Chen,Journal of translational medicine,2023
Recombinant human angiotensin-converting enzyme 2 plays a protective role in mice with sepsis-induced cardiac dysfunction through multiple signaling pathways dependent on converting angiotensin II to angiotensin 1–7 C Wu,Annals of Translational Medicine,2023
Bone morphogenetic protein 4 in perivascular adipose tissue ameliorates hypertension through regulation of angiotensinogen WJ Mu,Frontiers in cardiovascular medicine,2022
Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry Zilun L,Cell Metabolism,2022
PSGL1-deficient mice develop spontaneous pulmonary hypertension associated to systemic sclerosis Gonzalez-Tajuelo R, et al,Arthritis & Rheumatology,2019
The combination effects of Shen-Ling-Bai-Zhu on promoting apoptosis of transplanted H22 hepatocellular carcinoma in mice receiving chemotherapy. Xi S.et al,J Ethnopharmacol.,2016
The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,2016
Pitavastatin-attenuated cardiac dysfunction in mice with dilated cardiomyopathy via regulation of myocardial calcium handling proteins WEI HU et al,Acta pharmaceutica,2014
Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013

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Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.