Dog angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬血管緊張素Ⅱ(ANG-Ⅱ)酶聯免疫試劑盒（CSB-E15778c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ANG-Ⅱ含量。ANG - Ⅱ即血管緊張素Ⅱ，是腎素 - 血管緊張素系統的關鍵效應肽。它在血壓調節、水電解質平衡等方面發揮重要作用。研究機制上，主要通過與特異性受體結合，激活一系列細胞內信號通路，引發血管收縮、醛固酮釋放等生理反應。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研場景下評估藥物干預、疾病模型或生理狀態對ANG - Ⅱ水平的影響，例如犬類高血壓機制探索、腎臟疾病相關生物標志物分析或激素調控研究，為獸醫科研及動物模型開發提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

angiotension Ⅱ, angiotensin 2, ANG2
縮寫：

ANG-Ⅱ
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	98
	Range %	92-104
1:4	Average %	88
	Range %	86-93
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of dog ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	100	95-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.