Mouse activated protein C,APC ELISA Kit

Mouse activated protein C,APC ELISA Kit

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中文名稱：

小鼠活化蛋白C(APC)酶聯(lián)免疫試劑盒
貨號：

CSB-E09914m
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠活化蛋白C(APC)酶聯(lián)免疫試劑盒（CSB-E09914m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APC含量。APC（腺瘤性息肉病基因）是一種腫瘤抑制基因，其突變與結(jié)直腸癌的發(fā)生密切相關(guān)。研究顯示，APC的突變會導(dǎo)致Wnt/β-catenin信號通路異常激活，進(jìn)而影響炎癥信號參與結(jié)腸癌的發(fā)病過程。通過建立APC基因突變的小鼠腸癌模型，研究人員揭示了APC在結(jié)直腸癌發(fā)病機(jī)制中的關(guān)鍵作用，為開發(fā)新的治療策略提供了理論基礎(chǔ)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究，如凝血/纖溶系統(tǒng)調(diào)控機(jī)制探索、炎癥相關(guān)疾病動物模型評估、藥物干預(yù)對APC通路的影響研究等，尤其適用于從多類型樣本中系統(tǒng)性分析APC的動態(tài)變化，為心血管疾病、敗血癥等領(lǐng)域的科研工作提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

APC
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	90
	Range %	86-93
1:4	Average %	94
	Range %	90-97
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of mouse APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	95	91-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.602	2.588	2.595	2.433
2000	1.776	1.802	1.789	1.627
1000	1.093	1.078	1.086	0.924
500	0.715	0.698	0.707	0.545
250	0.457	0.434	0.446	0.284
125	0.304	0.312	0.308	0.146
62.5	0.244	0.232	0.238	0.076
0	0.164	0.160	0.162

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Knockdown of Clock gene induces thrombotic potential reduction by inhibiting α1-antitrypsin with promotion of fibronectin Shuting Cheng,Biological Rhythm Research,2020
Activated protein C targets immune cells and rheumatoid synovial fibroblasts to prevent inflammatory arthritis in mice Xue M, et al,Rheumatology,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.