Mouse Thrombospondin-2(THBS2) ELISA kit

Mouse Thrombospondin-2(THBS2) ELISA kit

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中文名稱：

小鼠血小板反應蛋白-2(THBS2)酶聯(lián)免疫試劑盒
貨號：

CSB-EL023488MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

小鼠血小板反應蛋白-2(THBS2)酶聯(lián)免疫試劑盒（CSB-EL023488MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的THBS2含量。THBS2是一個靶點。目前雖未給出其背景具體信息，但作為研究靶點，科學家可能圍繞它探索其在生物體內的作用機制，如與疾病發(fā)生、發(fā)展的關聯(lián)，在細胞信號傳導、增殖分化等過程中扮演的角色，以尋找潛在治療策略。試劑盒檢測范圍為6.25 ng/ml-400 ng/ml.，靈敏度為1.56 ng/ml.。科研人員可將其應用于心血管疾病模型、腫瘤微環(huán)境研究或組織修復機制探索等基礎研究領域，通過檢測不同病理狀態(tài)下THBS2的表達動態(tài)，為相關分子機制解析提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

Thbs2 ELISA Kit; Tsp2 ELISA Kit; Thrombospondin-2 ELISA Kit
縮寫：

THBS2
Uniprot No.：

Q03350
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates.
檢測范圍：

6.25 ng/ml-400 ng/ml.
靈敏度：

1.56 ng/ml.
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse THBS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-103
1:2	Average %	102
	Range %	97-106
1:4	Average %	89
	Range %	86-93
1:8	Average %	94
	Range %	89-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

400

2.366

2.454

2.410

2.230

200

1.523

1.633

1.578

1.398

100

0.974

0.958

0.966

0.786

0.603

0.632

0.618

0.438

0.421

0.425

0.423

0.243

12.5

0.298

0.286

0.292

0.112

6.25

0.233

0.250

0.242

0.062

0.179

0.180

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

最新研究進展：

THBS2是一種血小板的富含蛋白質，已知在多個生物過程中起到重要的作用。最近的研究表明，THBS2在多種癌癥的發(fā)展中發(fā)揮著作用，例如胃癌、乳腺癌、肺癌等。研究表明，THBS2與腫瘤的侵襲、轉移和血管新生有關。
功能：

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
基因功能參考文獻：
1. Data provide evidence that TSP2 transcription is suppressed by hypoxia through a mechanism that requires HIF-1alpha. PMID: 28789925
2. TWIST2 induces miR-221-3p expression and consequently suppresses its direct target, THBS2, in lymphatic metastasis cervical cancer. PMID: 29242498
3. TSP2 deficiency imparted a brittle phenotype on cortical bone. electron microscopy revealed less intensely stained collagen fibrils with altered morphology in the extracellular matrix assembled by osteoblasts on the anterior surface of TSP2-KO femora. PMID: 26272319
4. Enhanced levels of TSP-2 in the skin result in reduced susceptibility to chemically-induced skin carcinogenesis. PMID: 24507936
5. in the context of osteoblast differentiation, TSP2 promotes the assembly of a type-I collagen-rich matrix by facilitating pro-collagen processing. PMID: 23763373
6. The proposed mechanism for the shift in stem cell fate in fracture healing is that increased vascular density and hence oxygen availability in TSP2-null mice regulates differentiation. PMID: 23775935
7. The upregulation of TSP-2 mediated by increased oxidative stress contributes to angiogenesis dysfunction in diabetic bone marrow derived angiogenic cells. PMID: 23723366
8. TSP2 is a negative regulator of ischemic fracture healing, and in the absence of TSP2 bone regeneration is enhanced. PMID: 23280580
9. Using a TSP-2-knockout mouse model and cardiac cell transplantation, we found significantly reduced fibrosis and increased endothelial cell density in the peri-graft region. PMID: 22512900
10. the two TSP2 isforms are differentially modulated at injured and noninjured skeletal sites in an animal undergoing fracture healing. PMID: 22362307
11. TSP-2 has a protective role against cardiac inflammation, injury, and dysfunction in acute viral myocarditis. PMID: 22308237
12. astrocyte-derived TSP-2 is critical for the maintenance of physiological MMP-2 and MMP-9 levels during the foreign body response and contributes to the repair of the BBB PMID: 21704005
13. Endothelial nitric oxide synthase controls the expression of the angiogenesis inhibitor thrombospondin 2 PMID: 21949402
14. The secreted protein is an autocrine inhibitor of marrow stromal cell proliferation. PMID: 11874233
15. Megakaryocytes require this protein for normal platelet formation and function. PMID: 12732502
16. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling PMID: 12861025
17. TSP2-null mice provide an animal model to assist in the understanding of the molecular basis of spontaneous, premature softening of the uterine cervix. PMID: 14561659
18. Delay in expression of TSP2 and metalloproteinase 2 in the wounds of aged mice could contribute to their impaired rate of wound healing. PMID: 14996433
19. CD36 mediates the antiangiogenic effect of thrombospondin-2, which is modulated by histidine-rich glycoprotein PMID: 15748999
20. the absence of TSP2 protects against ovariectomy-induced bone loss by two complementary processes: increased formation and decreased resorption. PMID: 15979292
21. Mutations targeting intermodular interfaces or calcium binding destabilize the thrombospondin-2 signature domain. PMID: 18682400
22. TSP-2 was found to be present in some, but not all, annulus cells of the human annulus and the mouse annulus. PMID: 18718009
23. Taken together, our observations support a role for TSP2 as critical determinant of the brain response to biomaterials. PMID: 19020342
24. TSP-2 expression in the heart protects against age-dependent dilated cardiomyopathy. PMID: 19805649
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蛋白家族：

Thrombospondin family
數(shù)據(jù)庫鏈接：

KEGG: mmu:21826

STRING: 10090.ENSMUSP00000128308

UniGene: Mm.26688

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.