Mouse Semaphorin-3D(SEMA3D) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠臂板蛋白-3D(SEMA3D)酶聯免疫試劑盒（CSB-EL020983MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SEMA3D含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于研究SEMA3D在神經系統疾病、腫瘤微環境調控或免疫應答機制中的功能，尤其適合探索其在神經軸突導向、細胞遷移及組織再生等生物過程中的分子機制，為神經生物學、免疫學及腫瘤學等基礎研究領域提供高效的分析工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Sema3d ELISA Kit; Semaphorin-3D ELISA Kit
縮寫：

SEMA3D
Uniprot No.：

Q8BH34
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	99
	Range %	96-102
1:8	Average %	96
	Range %	89-103

回收率：

The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	98	92-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin.
基因功能參考文獻：
1. Authors did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome. PMID: 28334784
2. Data indicate that annexin A2 (AnxA2) regulates the secretion of semaphorin 3D (Sema3D) from pancreatic ductal adenocarcinoma (PDA) cells. PMID: 26243191
3. Semaphorin 3d requires neuropilin 1 or PI3K/Akt, whereas semaphorin 3e requires plexin D1 in directing endothelial motility. PMID: 24825896
4. In the absence of Sema3d, endothelial tubes form in a region that is normally avascular PMID: 23685842
5. Two proepicardial markers (Scleraxis and Semaphorin3D) were identified that genetically delineate heretofore uncharacterized proepicardial subcompartments. PMID: 22421048
6. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001
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亞細胞定位：

Secreted.
蛋白家族：

Semaphorin family
數據庫鏈接：

KEGG: mmu:108151

STRING: 10090.ENSMUSP00000030868

UniGene: Mm.89313