Mouse R-spondin-4(RSPO4) ELISA kit

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產品詳情

產品描述：

小鼠R脊椎蛋白4(RSPO4)酶聯免疫試劑盒（CSB-EL020553MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的RSPO4含量。RSPO4是重要靶點。它在胚胎發育、組織穩態維持等過程起關鍵作用。研究發現其通過與特定受體結合激活Wnt信號通路，影響細胞增殖與分化。在疾病方面，RSPO4異常表達和信號失調與腫瘤、皮膚病等發病機制相關。試劑盒檢測范圍為34.4 pg/mL-2200 pg/mL，適用于體外研究RSPO4在疾病模型中的動態變化，如基因編輯小鼠的蛋白表達驗證、病理狀態下信號通路的分子機制探索，或藥物干預后生物標志物的定量評估，為發育生物學、腫瘤學及代謝研究領域提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Rspo4R-spondin-4 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 4 ELISA kit; Cristin-4 ELISA kit; mCristin-4 ELISA kit; Roof plate-specific spondin-4 ELISA kit
縮寫：

RSPO4
Uniprot No.：

Q8BJ73
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

34.4 pg/mL-2200 pg/mL
靈敏度：

8.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Developmental Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-95
1:2	Average %	97
	Range %	93-102
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	95-101

回收率：

The recovery of mouse RSPO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	87	83-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
亞細胞定位：

Secreted.
蛋白家族：

R-spondin family
數據庫鏈接：

KEGG: mmu:228770

STRING: 10090.ENSMUSP00000041578

UniGene: Mm.159868