Mouse Protein Wnt-16(WNT16) ELISA kit

Mouse Protein Wnt-16(WNT16) ELISA kit

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中文名稱：

小鼠Wnt-16蛋白(WNT16)酶聯免疫試劑盒
貨號：

CSB-EL026132MO
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

小鼠Wnt-16蛋白(WNT16)酶聯免疫試劑盒（CSB-EL026132MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的WNT16含量。小鼠Wnt-16蛋白（WNT16）在骨骼穩態中起關鍵作用，其功能缺失會導致骨密度降低和骨折風險增加。WNT16通過調控成骨細胞功能影響骨骼發育，其調控機制涉及內含子2區域的啟動子活性與CPED1增強子區域的物理互作，可能影響骨骼穩態及骨質疏松癥治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于小鼠模型或細胞實驗中Wnt - 16蛋白表達水平的功能研究，例如探索骨骼發育異常機制、腫瘤微環境信號傳導或干細胞定向分化調控等科研場景；適用于基礎研究與藥物開發中Wnt信號通路的分子機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Wnt16 ELISA Kit; Protein Wnt-16 ELISA Kit
縮寫：

WNT16
Uniprot No.：

Q9QYS1
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Stem Cells
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	101-105
1:2	Average %	96
	Range %	93-99
1:4	Average %	89
	Range %	86-92
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of mouse WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	101-111
EDTA plasma (n=4)	92	89-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.379	2.465	2.422	2.268
2000	2.022	1.970	1.996	1.842
1000	1.450	1.560	1.505	1.351
500	0.865	0.899	0.882	0.728
250	0.531	0.527	0.529	0.375
125	0.375	0.383	0.379	0.225
62.5	0.288	0.278	0.283	0.129
0	0.158	0.150	0.154

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Wnt5a induces and maintains prostate cancer cells dormancy in bone Ren D, et al,Journal of Experimental Medicine,2018

功能：

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
基因功能參考文獻：
1. A large number of Mef2c targets overlapped with genes down-regulated by Wnt16 and Mef2c itself was transcriptionally repressed by Wnt16 suggesting that Mef2c plays a role in Wnt16-mediated transcriptional regulation. PMID: 29981832
2. that WNT16 plays a critical role for acquisition of both cortical and trabecular bone mass and strength PMID: 28013361
3. WNT16 antagonises excessive canonical WNT activation and protects cartilage in osteoarthritis. PMID: 27147711
4. Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone PMID: 26451596
5. Describe TGFbeta-Wnt16-Notch signaling conduit in the chondrocyte-like transformation of VSMCs and identify endogenous TGFbeta activity in MGP-null VSMCs as a critical mediator of chondrogenesis. PMID: 25614285
6. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
7. These findings suggest that WNT16 acting via canonical WNT signaling regulates mechanical strain-induced periosteal bone formation and bone size. PMID: 25521583
8. Osteoblast-derived WNT16 is a previously unreported key regulator of osteoclastogenesis and fracture susceptibility. PMID: 25306233
9. Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/beta-catenin pathway. PMID: 24037946
10. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Wnt family
數據庫鏈接：

KEGG: mmu:93735

STRING: 10090.ENSMUSP00000031681

UniGene: Mm.137403

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.