Mouse Plastin-2(LCP1) ELISA kit

Mouse Plastin-2(LCP1) ELISA kit

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中文名稱：

小鼠網素-2(LCP1)酶聯免疫試劑盒
貨號：

CSB-EL012824MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

小鼠網素-2(LCP1)酶聯免疫試劑盒（CSB-EL012824MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的LCP1含量。LCP1即淋巴細胞特異性蛋白1，在免疫系統中扮演重要角色。其表達異常和多種疾病相關，如腫瘤、炎癥等。研究發現它參與細胞的遷移、黏附等過程，通過與肌動蛋白相互作用影響細胞骨架的重組，為相關疾病診治提供新思路。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，特別適用于科研領域中對腫瘤轉移模型、免疫細胞功能研究以及相關分子機制探索的實驗需求，例如通過檢測藥物干預或基因編輯后LCP1的濃度變化，評估其對細胞遷移能力的調控作用。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Lcp1 ELISA Kit; Pls2 ELISA Kit; Plastin-2 ELISA Kit; 65 kDa macrophage protein ELISA Kit; L-plastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; LCP-1 ELISA Kit; pp65 ELISA Kit
縮寫：

LCP1
Uniprot No.：

Q61233
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	99
	Range %	94-103
1:4	Average %	84
	Range %	80-88
1:8	Average %	90
	Range %	86-94

回收率：

The recovery of mouse LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	106	101-113

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.612	2.537	2.575	2.434
800	2.142	2.056	2.099	1.958
400	1.471	1.567	1.519	1.378
200	0.874	0.905	0.890	0.749
100	0.534	0.579	0.557	0.416
50	0.290	0.279	0.285	0.144
25	0.229	0.249	0.239	0.098
0	0.144	0.137	0.141

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
基因功能參考文獻：
1. the actin-bundling protein L-plastin (LPL) is required for the perinatal development of alveolar macrophages. PMID: 27758872
2. Results identify L-plastin as a key effector of Mst1 and establish a novel mechanism linking a signaling intermediate to an actin-binding protein critical to T cell migration. PMID: 27465533
3. L-plastin modulates both T- and B-cell function during the germinal center reaction and the production of T-cell-dependent antibody responses. PMID: 23589339
4. L-plastin may participate in signaling that enables lymphocyte transmigration PMID: 21832165
5. LPL-dependent actin bundling facilitates the formation of lamellipodia and normal immunological synapses and thereby enables T cell activation. PMID: 21076065
6. Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. PMID: 20650888
7. We thus identify L-plastin as a molecule critical for CCR7-mediated t-cell motility but dispensable for early CCR7 signaling. PMID: 20194718
8. plastin plays a role in host defense distinguishes integrin signaling from cell adhesion and spreading. PMID: 12871642
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亞細胞定位：

Cytoplasm, cytoskeleton. Cell projection. Cell junction. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
數據庫鏈接：

KEGG: mmu:18826

STRING: 10090.ENSMUSP00000116271

UniGene: Mm.153911

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.