Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

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中文名稱：

小鼠血漿蛋白酶C1抑制劑(SERPING1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL021086MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠血漿蛋白酶C1抑制劑(SERPING1)酶聯(lián)免疫試劑盒（CSB-EL021086MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPING1含量。SERPING1是一種編碼參與補體級聯(lián)調節(jié)的高度糖基化血漿蛋白的基因，其編碼的蛋白質C1抑制劑抑制激活的第一補體成分的C1r和C1s，從而調節(jié)補體激活。其缺乏與遺傳性血管神經(jīng)性水腫（HANE）有關，并參與多種疾病的發(fā)生。試劑盒檢測范圍為5.6 ng/mL-360 ng/mL，靈敏度為1.4 ng/mL。適用于基礎醫(yī)學研究中探索免疫調節(jié)機制、炎癥性疾病模型建立、凝血功能相關實驗以及藥物干預效果評估等科研場景，為深入解析SERPING1在補體激活、血管通透性調節(jié)等生物學過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
縮寫：

SERPING1
Uniprot No.：

P97290
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5.6 ng/mL-360 ng/mL
靈敏度：

1.4 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	100
	Range %	95-105
1:2000	Average %	104
	Range %	101-107
1:4000	Average %	83
	Range %	80-86
1:8000	Average %	98
	Range %	95-103

回收率：

The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-92
EDTA plasma (n=4)	96	93-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
360	2.559	2.569	2.564	2.461
180	2.253	2.298	2.276	2.173
90	1.822	1.843	1.833	1.730
45	1.079	1.098	1.089	0.986
22.5	0.602	0.634	0.618	0.515
11.25	0.331	0.351	0.341	0.238
5.6	0.205	0.220	0.213	0.110
0	0.102	0.104	0.103

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
基因功能參考文獻：
1. C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
2. Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
3. In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
4. C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
5. In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060
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亞細胞定位：

Secreted.
蛋白家族：

Serpin family
數(shù)據(jù)庫鏈接：

KEGG: mmu:12258

STRING: 10090.ENSMUSP00000023994

UniGene: Mm.38888

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.