Mouse Pentosidine ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09417m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Pentosidine含量。Pentosidine，即戊糖素，是一種高級糖基化終產(chǎn)物（AGEs），其生成與人體衰老、糖尿病、尿毒癥等疾病密切相關(guān)。研究顯示，乳酸菌發(fā)酵物能有效抑制戊糖素生成，具有預防或治療相關(guān)疾病的作用。其機制在于乳酸菌發(fā)酵物中的有效成分能夠減少戊糖素的生成，從而降低與AGEs相關(guān)的疾病風險。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，廣泛應(yīng)用于糖尿病模型研究、衰老相關(guān)氧化應(yīng)激機制探索、代謝性疾病中蛋白質(zhì)修飾分析等科研領(lǐng)域，為體外實驗提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Pentosidine
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-103
1:200	Average %	85
	Range %	80-90
1:400	Average %	93
	Range %	89-98
1:800	Average %	99
	Range %	94-105

回收率：

The recovery of mouse pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.