Mouse Matrix Gla Protein(MGP)ELISA Kit

Mouse Matrix Gla Protein(MGP)ELISA Kit

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中文名稱：

小鼠基質(zhì)γ羧基谷氨酸蛋白(MGP)酶聯(lián)免疫試劑盒
貨號：

CSB-E16540m
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠基質(zhì)γ羧基谷氨酸蛋白(MGP)酶聯(lián)免疫試劑盒（CSB-E16540m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MGP含量。小鼠基質(zhì)γ羧基谷氨酸蛋白（MGP）是一種維生素K依賴性蛋白，主要存在于骨骼、軟骨、心臟、腎臟和肺等器官中，具有抑制鈣離子在軟組織異常沉積的作用，與多種疾病如骨質(zhì)疏松、動脈硬化等密切相關(guān)。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，科研人員可通過探究MGP在血管鈣化模型、骨代謝疾病動物實驗中的動態(tài)變化，或結(jié)合細胞實驗研究其在礦化相關(guān)信號通路中的作用機制，為心血管及骨骼系統(tǒng)疾病的分子機制研究提供可靠工具。該產(chǎn)品嚴格遵循科研用途設計，適用于基礎研究中的蛋白表達定量及病理生理機制分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Mgp ELISA Kit; Mglap ELISA Kit; Matrix Gla protein ELISA Kit; MGP ELISA Kit
縮寫：

MGP
Uniprot No.：

P19788
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	93-99
1:200	Average %	87
	Range %	85-89
1:400	Average %	91
	Range %	85-97
1:800	Average %	104
	Range %	102-106

回收率：

The recovery of mouse MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	87	85-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.432	2.468	2.450	2.337
1000	2.169	2.150	2.160	2.047
500	1.704	1.735	1.720	1.607
250	1.115	1.136	1.126	1.013
125	0.568	0.598	0.583	0.470
62.5	0.344	0.368	0.356	0.243
31.25	0.226	0.236	0.231	0.118
0	0.114	0.112	0.113

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
基因功能參考文獻：
1. We propose MGP as a strong candidate for the regulation of stiffness in glaucoma. MGP further illustrates the presence of a common function affecting key glaucomatous parameters in the front and back of the eye, and thus offers the possibility for a sole therapeutic target for the disease. PMID: 27593913
2. the nasal septum to be abnormally mineralized and shortened in Mgp(-/-) mice. Transgenic restoration of Mgp expression in chondrocytes fully corrected the craniofacial anomalies caused by MGP deficiency, suggesting a local role for MGP in the developing nasal septum. PMID: 28487368
3. loss of MGP causes dysregulation of early endothelial differentiation. PMID: 26364300
4. Matrix Gla protein limits pulmonary arteriovenous malformations in ALK1 deficiency. PMID: 25614167
5. expression of MGP and OCN increased gradually in the murine developing tibial epiphysis, and the two mineral-associated proteins may occur at the same location during a particular period, but at different levels. PMID: 24956112
6. Matrix Gla's restricted expression to glaucoma-associated tissues from anterior and posterior segments suggests its involvement in the development of the disease. PMID: 25711639
7. Elastin haploinsufficiency impedes the progression of arterial calcification in MGP-deficient mice. PMID: 23857752
8. The arterial calcification, not MGP deficiency itself, causes the low bone mass phenotype in Mgp-/- mice. PMID: 23994172
9. Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin. PMID: 24036114
10. MGP is a multi-functional inhibitor of normal and abnormal angiogenesis that may function by coordinating with both Notch and BMP signaling pathways PMID: 23110920
11. Mgp gene deletion may have a role in arteriovenous malformations PMID: 21765215
12. role in vascular calcification in vivo PMID: 12391016
13. MGP is a known inhibitor of mineralization, and mice deficient in Mgp show severe vascular calcification and premature bone mineralization. PMID: 16612082
14. Inorganic phosphate(Pi) regulates MGP expression in growth plate chondrocytes, thereby suggesting a key role for Pi and ERK1/2 in the regulation of bone formation. PMID: 17068135
15. PTH regulates MGP via both PKA- and ERK-dependent pathways. PMID: 17407158
16. Matrix GLA protein is an inhibitory morphogen in pulmonary vascular development PMID: 17670744
17. MGP in Abcc6-/- mice is largely in inactive form and is unable to prevent the unwanted mineralization of connective tissues in PXE. PMID: 17942075
18. Extensive hypomineralization was observed in dentin and alveolar bone from transgenic mice expressing matrix Gla protein when compared with that in wild-type tissues. Mineralization was virtually absent in tooth root dentin and cellular cementum. PMID: 18719210
19. Oral anticoagulant treatment decreased local expression of MGP. PMID: 19350115
20. Inorganic phosphate regulates MGP expression in osteoblasts through the ERK1/2-Fra-1 pathway. PMID: 19419315
21. Heat shock protein 70 enhances vascular bone morphogenetic protein-4 signaling by binding matrix Gla protein. PMID: 19661459
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亞細胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
數(shù)據(jù)庫鏈接：

KEGG: mmu:17313

STRING: 10090.ENSMUSP00000032342

UniGene: Mm.243085

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.