Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

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中文名稱：

小鼠溶酶體Pro-X羧肽酶(PRCP)酶聯(lián)免疫試劑盒
貨號：

CSB-EL018636MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠溶酶體Pro-X羧肽酶(PRCP)酶聯(lián)免疫試劑盒（CSB-EL018636MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PRCP含量。小鼠溶酶體Pro-X羧肽酶（PRCP）是一種在溶酶體內(nèi)發(fā)揮作用的蛋白酶，參與蛋白質(zhì)的降解和代謝。其功能與調(diào)節(jié)血壓和電解質(zhì)平衡有關，研究其機制有助于理解高血壓等疾病的發(fā)生。試劑盒檢測范圍為37.5 pg/mL-2400 pg/mL，適用于體外培養(yǎng)細胞模型中PRCP分泌調(diào)控研究、基因敲除動物模型的蛋白表達驗證，或疾病狀態(tài)下溶酶體功能相關分子機制的探索性分析，為心血管生物學、細胞代謝研究及溶酶體相關疾病機制研究提供可靠的定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Prcp ELISA Kit; Lysosomal Pro-X carboxypeptidase ELISA Kit; EC 3.4.16.2 ELISA Kit; Proline carboxypeptidase ELISA Kit; Prolylcarboxypeptidase ELISA Kit; PRCP ELISA Kit
縮寫：

PRCP
Uniprot No.：

Q7TMR0
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

37.5 pg/mL-2400 pg/mL
靈敏度：

9.37 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	98
	Range %	92-103
1:4	Average %	93
	Range %	89-98
1:8	Average %	91
	Range %	87-96

回收率：

The recovery of mouse PRCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	94	93-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.542	2.520	2.531	2.377
1200	2.017	1.955	1.986	1.832
600	1.427	1.321	1.374	1.220
300	0.722	0.781	0.752	0.598
150	0.465	0.455	0.460	0.306
75	0.322	0.346	0.334	0.180
37.5	0.249	0.248	0.249	0.095
0	0.154	0.153	0.154

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
基因功能參考文獻：
1. Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP. PMID: 28160049
2. activity and expression in plasma and lung tissue stays unaffected in lung ischemia-reperfusion injury PMID: 27437782
3. This study demonistrated that PRCP gene is broadly expressed in the brain. PMID: 24161824
4. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
5. PRCP is an important regulator of energy and glucose homeostasis since its deletion significantly improves metabolic parameters in mice exposed to both standard chow diet and high-fat diet PMID: 22454290
6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
7. This study showed that PRCP mutant mice have a significant decrease in fat mass, although an increase in lean mass was also observed; reduced leptin levels and increased energy expenditure were also found. PMID: 22202165
8. Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis. PMID: 21297000
9. PRCP is an important component of melanocortin signaling and weight maintenance via control of active alpha-MSH1-13 levels. PMID: 19620781
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亞細胞定位：

Lysosome.
蛋白家族：

Peptidase S28 family
數(shù)據(jù)庫鏈接：

KEGG: mmu:72461

STRING: 10090.ENSMUSP00000075429

UniGene: Mm.389969

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.