Mouse Leukotriene B4,LT-B4 ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠白三烯B4(LTB4)酶聯免疫試劑盒（CSB-E08034m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的LT-B4含量。LT-B4，即白三烯B4，是一種由花生四烯酸代謝生成的生物活性物質，具有高度炎癥反應性。它在急性髓系白血病（AML）患者中表達水平異常，與不良預后相關，可能通過調控免疫微環境、促進腫瘤增殖和侵襲等機制影響AML的發生發展。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外實驗中對炎癥機制、免疫調節或藥物干預效果的評估研究；為炎癥性疾病模型研究、免疫調控機制探索等科研項目提供可靠的實驗數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

LT-B4
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	86-97
1:4	Average %	95
	Range %	91-99
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of mouse LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-101
EDTA plasma (n=4)	93	89-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.