Mouse Interleukin 35(IL-35) ELISA Kit

Mouse Interleukin 35(IL-35) ELISA Kit

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中文名稱：

小鼠白介素35(IL-35)酶聯(lián)免疫試劑盒
貨號：

CSB-E13145m
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠白介素35(IL-35)酶聯(lián)免疫試劑盒（CSB-E13145m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL-35含量。IL-35是IL-12家族的新成員，由EBI3和p35亞基組成。它在免疫調(diào)節(jié)中發(fā)揮關鍵作用，與IL12B形成IL-12細胞因子或與EBI3/IL27B形成IL-35細胞因子。IL-35在多種炎癥和腫瘤中表達異常，其作用機制涉及調(diào)節(jié)T細胞和自然殺傷細胞反應，以及誘導干擾素γ的產(chǎn)生。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外培養(yǎng)細胞因子分泌分析、實驗動物模型血清/組織IL-35動態(tài)監(jiān)測等場景；為研究IL-35在免疫調(diào)控機制、疾病模型中的功能解析及藥物干預效果評價提供了高效工具，廣泛應用于免疫學、腫瘤學及炎癥相關基礎研究領域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-35
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-100
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	96	91-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.461	2.391	2.426	2.235
500	2.073	1.965	2.019	1.828
250	1.669	1.577	1.623	1.432
125	1.272	1.183	1.228	1.037
62.5	0.814	0.804	0.809	0.618
31.2	0.567	0.525	0.546	0.355
15.6	0.335	0.314	0.325	0.134
0	0.194	0.188	0.191

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Dendrobium huoshanense polysaccharide inhibits NSCLC proliferation and immune evasion via FXR1-IL-35 axis signaling pathway X Zhu, G Yin, J Xu, X Tang, F Yu,Journal of Natural Medicines,2025
A Silk Fibroin Nanoparticle Hydrogel Loaded With NK1R Antagonist Has Synergistic Anti‐Inflammatory and Reparative Effects on Dry Eye Disease B Gong, Y Liu, H Li, X Ju, D Li, Y Zou, X Guo,Advanced Science,2025
IL-35 may prevent the exacerbation of aspiration pneumonia involving Porphyromonas gingivalis by suppressing IL-17 production S Kawamura, H Goto, T Kikuchi, T Okabe,American Journal of Pathology,2024
Populational change of CD4+CD25+Treg cells is responsible for the synergistic effect of the combination of RAMP2 with baicalin in treating recurrent spontaneous abortion mouse models C Chen,/,2024
Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion Liu J, et al,Nature Communications,2019
Interleukin-35 expression protects against cigarette smoke-induced lung inflammation in mice Pan X, et al,Biomedicine & pharmacotherapy,2018
Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation Liu X.et al,Cell Physiol Biochem,2018
Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury Xia Yang.et al, Iranian Journal of Allergy, Asthma and Immunology,2017
Isorhamnetin attenuates collagen-induced arthritis via modulating cytokines and oxidative stress in mice Wang X. et al,Int J Clin Exp Med,2015
The role of CCR7 in allergic airway inflammation induced by house dust mite exposure /,Cellular Immunology,2012

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.