Mouse Interleukin 35(IL-35) ELISA Kit

產品詳情

文獻引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠白介素35(IL-35)酶聯免疫試劑盒（CSB-E13145m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL-35含量。IL-35是IL-12家族的新成員，由EBI3和p35亞基組成。它在免疫調節中發揮關鍵作用，與IL12B形成IL-12細胞因子或與EBI3/IL27B形成IL-35細胞因子。IL-35在多種炎癥和腫瘤中表達異常，其作用機制涉及調節T細胞和自然殺傷細胞反應，以及誘導干擾素γ的產生。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外培養細胞因子分泌分析、實驗動物模型血清/組織IL-35動態監測等場景；為研究IL-35在免疫調控機制、疾病模型中的功能解析及藥物干預效果評價提供了高效工具，廣泛應用于免疫學、腫瘤學及炎癥相關基礎研究領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-35
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-100
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	96	91-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.