Mouse Interleukin 23,IL-23 ELISA Kit

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中文名稱：

小鼠白介素23(IL-23)酶聯免疫試劑盒
貨號：

CSB-E08463m
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產品描述：

小鼠白介素23(IL-23)酶聯免疫試劑盒（CSB-E08463m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-23含量。IL-23是一種細胞因子，在免疫系統中發揮重要作用。它能夠調節Th17細胞的生成與功能，與自身免疫疾病、炎癥反應密切相關。目前針對IL-23的研究聚焦于其信號通路及作用機制，以開發相關治療藥物來改善炎癥和免疫異常狀況。試劑盒檢測范圍為7.8 pg/ml-500 pg/ml，適用于科研領域中對感染性疾病模型、炎癥反應機制、免疫調節研究的IL - 23動態監測，也可用于評估藥物或生物制劑對IL - 23通路的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

IL-23
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/ml-500 pg/ml
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	95
	Range %	89-100
1:4	Average %	93
	Range %	84-97
1:8	Average %	106
	Range %	97-110

回收率：

The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	101	94-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.868	2.689	2.779	2.654
250	1.519	1.586	1.553	1.428
125	0.746	0.700	0.723	0.598
62.5	0.434	0.456	0.445	0.320
31.2	0.314	0.301	0.308	0.183
15.6	0.207	0.202	0.205	0.080
7.8	0.153	0.159	0.156	0.031
0	0.127	0.123	0.125

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

QingChang-XiaoPi Decoction Ameliorates Intestinal Inflammation of Ulcerative Colitis by Regulating the Pathogenicity of Th17 Cells R Jia,Phytomedicine,2024
Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF‐α and IL‐23p19 bioactivities J Wang,Clinical and translational medicine,2024
Tanshinone IIA, a component of the self‐made Xiao‐Yin decoction, ameliorates psoriasis by inhibiting IL‐17/IL‐23 and PTGS2/NF‐κB/AP‐1 pathways G Zou,Skin research and technology,2024
IFN-r induced by IL-12 administration prevents diabetes by inhibiting pathogenic IL-17 production in NOD mice Jun Zhang et al,Journal of Autoimmunity,2012

產品評價

平均分:

5.0分 - 1 個評價

樣品類型：組織勻漿液海馬組織

樣品信息：小鼠

稀釋比：1:50

產品評價：我用CSB-E08463m分別檢測了磁刺激前后小鼠海馬組織勻漿中IL-23水平，標曲結果良好，發現BTBR小鼠IL-23水平高于C57小鼠，磁刺激后IL-23水平降低，與預期結果一致。試劑盒操作方便。

By 王老師

相關問答

■ 常見問題解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Mouse Interleukin 23,IL-23 ELISA Kit

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