Human Interleukin 23,IL-23 ELISA Kit

Human Interleukin 23,IL-23 ELISA Kit

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文獻引用 (18) 說明書下載

中文名稱：

人白介素23(IL-23)酶聯免疫試劑盒
貨號：

CSB-E08461h
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產品描述：

人白介素23(IL-23)酶聯免疫試劑盒（CSB-E08461h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-23含量。IL-23是重要治療靶點。它是一種促炎細胞因子，在自身免疫病發病機制中起關鍵作用。研究發現阻斷IL-23信號通路可調節免疫反應，有望為銀屑病、炎癥性腸病等疾病治療帶來新方法，目前相關藥物研發是熱點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，科研人員可將其用于免疫機制研究、炎癥性疾病模型分析、藥物靶點篩選等基礎研究領域，特別適用于評估實驗動物或細胞模型中IL - 23的表達變化及其在病理生理過程中的調控作用。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

IL-23
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	98
	Range %	92-106
1:4	Average %	94
	Range %	90-98
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	93	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.608	2.507	2.558	2.415
200	1.865	1.764	1.815	1.672
100	1.207	1.186	1.197	1.054
50	0.801	0.790	0.796	0.653
25	0.437	0.448	0.443	0.300
12.5	0.301	0.312	0.307	0.164
6.25	0.228	0.234	0.231	0.088
0	0.142	0.143	0.143

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

ANXA6 Overexpression Causes Abnormal Decidual Macrophage-Trophoblast Crosstalk in Recurrent Spontaneous Abortion X Chen, XY Li, XQ Ma, Y Zhang,International Journal of Biological Sciences,2025
Особенности цитокиновой продукции у пациентов с бактериальной пневмонией, протекающей на фоне острой респираторной вирусной инфекции ВК Парфенюк, НВ Бондарь,Российский медико-биологический вестник имени академика ИП Павлова,2025
Profiling of IgG N-glycosylation for axial spondyloarthritis and other rheumatic diseases X Xu, Z Chen, M Song, Z Hou,Arthritis Research & Therapy,2025
METTL14-m6A-FOXO3a axis regulates autophagy and inflammation in ankylosing spondylitis Y Chen,Clinical immunology,2023
Study of serum some immune facter of CD4 and IL-23 indicatore on thalassemia patients in Abn Albaladiu hospital in Baghdad IH Mohammed,AIP Publishing LLC,2023
Interleukin-17, Interleukin-23 and Alkalinephosphatase Levels in Patients with Helicobacter pylori Infection KIA Mohammed,South Asian Res J Bio Appl Biosci,2022
Protective Roles of tRNA‐Derived Small RNA tRF‐Ile‐AAT‐019 in Pathological Progression of Psoriasis Z Jinrong,Experimental Dermatology,2022
Serum Levels of Il-12 and Il-23 in Breast Cancer Patients Infected with Toxoplasma gondii: A Case-Control Study Wafaa Mohammed ElZefzafy,iranian journal parasitology,2020
H19 increases IL-17A/IL-23 releases via regulating VDR by interacting with miR675-5p/miR22-5p in ankylosing spondylitis Zhang X, et al,Molecular Therapy-Nucleic Acids,2019
Comparison of pleural effusion features and biomarkers between talaromycosis and tuberculosis in non-human immunodeficiency virus-infected patients Qiu Y, et al,Bmc Infectious Diseases,2019
Genistein suppresses psoriasis-related inflammation through a STAT3-NF-κB-dependent mechanism in keratinocytes Wang A, et al,International Immunopharmacology,2019
Evaluation of IL-23 concentration in a miscarriages woman Infected with Toxoplasma gondii Raad Ajam Sayal, et al,/,2018
The Association of Severe Toxoplasmosis and Some Cytokine Levels in Breast Cancer Patients Maha Mustafa Assim.et al,Iraqi Journal of Science,2018
Up-regulated lncRNA-MSX2P1 promotes the growth of IL-22-stimulated keratinocytes by inhibiting miR-6731-5p and activating S100A7 Meng Qiao.et al,Experimental Cell Research,2018
Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with β-cell autoantigens. Badal D.et al,Indian J Med Res. ,2017
Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface Li N.et al,Braz J Med Biol Res,2017
Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23 Chen G.et al,PLoS One.,2016
Distinct intrathecal interleukin-17/interleukin-6 activation in anti-N-methyl-d-aspartate receptor encephalitis Byun J I.et al,Journal of Neuroimmunology,,2016

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■ 常見問題解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit
If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

I am interested in this kit. I would like to know which subunit of IL-23 can be detected by the kit.
Please provide a detailed information about kit’s components:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

Thanks for your inquiry.
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of an IL-12p40 subunit (that is shared with IL-12 ) and the IL-23p19 subunit.
CSB-E08461h
This kit detects IL23（it is compound）.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is a dimer of recombinant proteins expressed by eukaryotic cells in vitro.

Thank you for your assistance.
Is the 293 protein you are referring the IL-23 p19 subunit?
Because you just mention the immunogen is 293 cell-derived recombinant protein. Please clarify, what is the 293 cell-derived protein specifically? For example, is it the IL-23 p19 subunit?
It is good to know, if the antibodies bind to the p40 unit or the p19 subunit?
Looking forward to receiving your feedback.

Thanks for your further inquiry. There are 2 antibodies in the kit, so there are 2 immunogens. They are designed against the 2 subunits of the compound.
Sorry we can not disclose which antibody is specific to which protein.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.