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  4. Mouse Interleukin 12,IL-12/P70 ELISA KIT

Mouse Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名稱：
    小鼠白介素12(IL-12/P70)酶聯免疫試劑盒
  • 貨號：
    CSB-E04600m-IS
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠白介素12(IL-12/P70)酶聯免疫試劑盒（CSB-E04600m-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-12/P70含量。IL-12/P70是重要細胞因子。其背景上，IL-12參與免疫調節。研究機制方面，它能激活T細胞和NK細胞，增強免疫細胞活性、促進炎癥反應，在腫瘤免疫、抗感染等領域是研究靶點，有望用于開發新型治療方案。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，靈敏度為1.95 pg/mL。該產品可精準檢測小鼠樣本中IL-12/P70異源二聚體含量，廣泛應用于免疫機制研究、炎癥模型評估、疫苗佐劑效果評價等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    N/A
  • 縮寫：
    IL-12/P70
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    83-95

    1:2

    Average %

    93

    Range %

    88-99

    1:4

    Average %

    97

    Range %

    91-104

    1:8

    Average %

    93

    Range %

    86-99

  • 回收率：

    The recovery of mouse IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    83-98

    EDTA plasma (n=4)

    99

    92-107

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.862

    2.734

    2.798

    2.603

    250

    2.052

    1.967

    2.010

    1.815

    125

    1.366

    1.358

    1.362

    1.167

    62.5

    0.885

    0.860

    0.873

    0.678

    31.2

    0.551

    0.541

    0.546

    0.351

    15.6

    0.400

    0.383

    0.392

    0.196

    7.8

    0.303

    0.294

    0.298

    0.103

    0

    0.198

    0.193

    0.195

    　

  • 數據處理：
  • 貨期：
    3-5 working days

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