Pig Interleukin 12,IL-12/P70 ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬白介素12(IL-12/P70)酶聯免疫試劑盒（CSB-E13288p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL12/P70含量。IL12/P70是一種重要的靶點。它在免疫系統中發揮關鍵作用，能夠調節免疫細胞功能，參與機體炎癥反應和免疫應答過程。相關研究主要聚焦于其信號傳導通路和與疾病的關聯機制，有望為炎癥性疾病、腫瘤等的治療提供新策略。試劑盒檢測范圍為0.45 ng/mL-30 ng/mL，靈敏度為0.11 ng/mL。適用于豬類動物模型中的免疫調控機制研究、疫苗效力評估、感染性疾病或炎癥反應模型中細胞因子網絡的動態分析；適用于畜牧獸醫研究、免疫毒理學實驗及生物制劑開發等領域的定量分析需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
縮寫：

IL-12/P70
Uniprot No.：

Q29053
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.45 ng/mL-30 ng/mL
靈敏度：

0.11 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	84
	Range %	80-89
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
亞細胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
數據庫鏈接：

KEGG: ssc:397053

STRING: 9823.ENSSSCP00000012501

UniGene: Ssc.13