Mouse ICOS ligand(ICOSLG) ELISA kit

Mouse ICOS ligand(ICOSLG) ELISA kit

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中文名稱：

小鼠ICOS配體(ICOSLG)酶聯免疫試劑盒
貨號：

CSB-EL010958MO
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

小鼠ICOS配體(ICOSLG)酶聯免疫試劑盒（CSB-EL010958MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ICOSLG含量。ICOSLG（誘導型T細胞共刺激配體）是一種跨膜糖蛋白，屬于B7配體家族，廣泛表達于多種抗原呈遞細胞和非淋巴細胞。它作為T細胞特異性細胞表面受體ICOS的配體，在T細胞的活化、增殖、分化和細胞因子產生中起重要作用，同時促進B細胞分泌抗體。ICOSLG的研究機制涉及其在T/B細胞相互作用中的正反饋調節，以及與PI3K-AKT通路在腫瘤免疫治療中的作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于免疫調節機制研究、疾病模型驗證或藥物干預實驗等科研場景。通過檢測不同生理病理條件下 ICOSLG 的表達動態，可為免疫微環境調控、細胞信號通路探索及治療靶點開發提供可靠數據支持，滿足免疫學基礎研究與轉化醫學實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Icoslg ELISA Kit; B7h2 ELISA Kit; B7rp1 ELISA Kit; IcoslICOS ligand ELISA Kit; B7 homolog 2 ELISA Kit; B7-H2 ELISA Kit; B7-like protein Gl50 ELISA Kit; B7-related protein 1 ELISA Kit; B7RP-1 ELISA Kit; LICOS ELISA Kit; CD antigen CD275 ELISA Kit
縮寫：

ICOSLG
Uniprot No.：

Q9JHJ8
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	100
	Range %	95-104
1:4	Average %	97
	Range %	92-110
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of mouse ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.417	2.552	2.485	2.322
2000	2.104	2.076	2.090	1.927
1000	1.450	1.404	1.427	1.264
500	0.765	0.723	0.744	0.581
250	0.439	0.471	0.455	0.292
125	0.321	0.342	0.332	0.169
62.5	0.243	0.229	0.236	0.073
0	0.169	0.157	0.163

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
基因功能參考文獻：
1. study confirms the importance of ICOSL shedding in ICOS/ICOSL function and expression and it identifies ADAM10 as the most important sheddase for controlling ICOSL levels PMID: 28814605
2. results showed that monocyte-derived osteoclast (OC)-like cells (MDOCs) express B7h during their differentiation, and that B7h triggering reversibly inhibits OC differentiation and function both in vitro and in vivo PMID: 27798154
3. study concludse that lymphopenia-driven autoimmunity and the development of Tfh17 cells in Rasgrp1-deficient mice occur independently of CD275(B7-H2) PMID: 27183569
4. The expression of ICOSL in the cornea and the ICOS-mediated induction of Foxp3+ CD4+ regulatory T cells may contribute to successful corneal allograft survival. PMID: 28002569
5. this study shows that sICOSL in the serum of healthy donors increases in an age-dependent manner and that the matrix metalloproteinase inhibitor (MMPI) could suppress sICOSL production PMID: 27138044
6. There was a clearly positive correlation between the presence of IL-17-producing cells and ICOS expression in ICOSL KO mice; it also showed that Th17 was involved in the pathological tissue remodeling in liver fibrosis induced by schistosomiasis. PMID: 25590646
7. Selective ablation of ICOSL in CD11c+ cells, but not in B cells, dramatically ameliorates kidney and lung inflammation in lupus-prone transgenic mice. PMID: 25786178
8. restoring control of the T follicular helper-germinal center B-cell axis by blocking the ICOS-ICOSL pathway reduced the development of atherosclerosis and the formation of tertiary lymphoid organs. PMID: 25552357
9. ICOSL is a molecular linkage between T-B interactional dynamics and positive selection for high-affinity bone-marrow plasma cell formation; study reveals a pathway by which follicular T-helper cells control the quality of long-lived humoral immunity PMID: 25317561
10. the costimulatory ligand ICOS ligand (ICOSL) is selectively downregulated on the surface of B cells in an ADAM17-specific manner, although it is not proteolitically processed by recombinant ADAM17 in vitro. PMID: 25108021
11. the B7h-ICOS interaction may modulate the spread of cancer metastases PMID: 24729612
12. Findings indicate the separable roles of delivery of antigens and ICOS-L by cognate B cells for follicular Th (Tfh) cell maturation and function. PMID: 24610013
13. Data indicate that the signals provided by ICOSL-expressing B cells to Teff cells and Tfh cells are necessary for the development of arthritis. PMID: 24449576
14. ICOS-L maintains tolerance at the fetomaternal interface. PMID: 23578385
15. In cardiac transplantation apoptosis of CD8(+) T cells in recipient draining lymph nodes was enhanced by pretreatment with donor specific transfusion and impaired ICOS/B7h allorecognition. PMID: 23498793
16. Data suggest that knockdown of Icosl in mouse leukemic cells may significantly enhance graft versus leukemia effect after allogeneic bone marrow transplantation. PMID: 22732700
17. lineage-specific transgenic expression on dendritic and plasma cells generates different outcome upon ICOS costimulation PMID: 22686515
18. Results indicate that ICOSLG blockade may enhance cytotoxity in allogeneic mixed lymphocyte-hematologic neoplasm cell reactions. PMID: 21958057
19. Induction of IL-10 producing regulatory T cells mainly relied on the inducible costimulator ligand (ICOSL)/ICOS axis PMID: 22154192
20. ICOS/B7h signal plays an important role in direct allorecognition, eliciting allogeneic responses in vitro. PMID: 22172879
21. Findings suggest that the noncanonical NF-kappaB pathway regulates the development of Tfh cells by mediating ICOSL gene expression in B cells. PMID: 21768353
22. A role for ICOS costimulation in the maintenance of effector memory but not central memory CD4 T cells. PMID: 21364749
23. demonstrate that inducible costimulator (ICOS) provides a critical early signal to induce the transcription factor Bcl6, and Bcl6 then induces CXCR5, the canonical feature of follicular helper CD4(+) T cells PMID: 21636296
24. expression is essential for allergic airway hyperresponsiveness PMID: 21402623
25. The data suggest that ICOSL plays a significant role in immunoregulation and protective immunity against Chlamydia infections PMID: 20190137
26. These studies demonstrate that T(R) cells and the ICOS-ICOS-ligand signaling pathway are critically involved in respiratory tolerance and in downregulating pulmonary inflammation in asthma. PMID: 12145647
27. The involvement of the B7h-Icos costimulatory pathway has been demonstrated in the development and progression of collagen-induced arthritis. PMID: 12370365
28. ICOS-L is a ligand for ICOS and plays an important functional role in the activation of memory T cells by endothelial cells [review] PMID: 12456022
29. While interactions of B7RP-1 with ICOS have no effect on the migration of T cells into B cell follicles, they are required for Th1 and Th2 cells to support fulminant B cell responses in vivo. PMID: 12594252
30. B7-H2 on B cells is important for recruiting T-cell help via its interaction with ICOS and plays a critical role in costimulating humoral immune responses. PMID: 12714510
31. data suggest that ICOS/B7RP-1 interactions may not affect the organogenesis, but involve in the functional development of Peyer's patches PMID: 12853164
32. the ICOS-B7h pathway has roles in regulating alloimmune responses in vivo PMID: 12865411
33. Th cells, when activated with B7h-deficient APC, exhibited reduced proliferation and IL-2 production. PMID: 14615582
34. ICOS-B7h has a role in transplantation tolerance to MHC class II mismatched skin grafts PMID: 15636609
35. Experiments with mice genetically engineered for the absence of B7-related protein 1 (B7RP-1KO) demonstrate that efficient T helper 2 (TH2) cell sensitization and effector function occur in the absence of the ICOS-B7RP-1 costimulatory pathway. PMID: 15728513
36. ICOS-B7h pathway is critical in the activation of effector/memory T cells that are necessary for the progression of chronic rejection. PMID: 15880041
37. The B7RP-1/ICOS interaction plays an essential role in the development of CXCR-positive helper T cells in vivo, which preferentially migrate to the germinal center B cell zone where they provide cognate help to B cells. PMID: 16081804
38. ICOSL is upregulated in nephritic glomeruli, where it locally reduces accumulation of T cells and macrophages and attenuates renal injury. PMID: 16540559
39. critical role played by ICOS-L-expressing and interleukin 10-producing dendritic cells from Chlamydia-infected mice in the infection-mediated inhibition of allergic responses PMID: 16621988
40. B7h shedding defines a novel immunoregulatory mechanism by which toll-like receptor (TLR)7/8 and TLR9 signals can enhance T cell help during antibody responses. PMID: 16887997
41. These results suggest that the ICOS/B7RP-1 interaction plays a critical role in the pathogenesis of uveitis. PMID: 17039566
42. The generated a 3T3 cellular library retrovirally expressing mutants of the murine costimulatory B7h gene. Screening of this unbiased cellular library identified six residues of murine B7h that are critical for binding to the ICOS receptor. PMID: 18294651
43. a novel mechanism for the regulation of ICOSL expression in vivo and suggest that the ICOS costimulatory pathway is subject to negative feedback regulation by ICOSL down-regulation in response to ICOS expression. PMID: 18390703
44. Levels of follicular T helper cells and germinal center B cells in two different models of autoimmunity are shown to be dependent on the maintenance of the inducible T-cell co-stimulator (ICOS)/B7-related protein-1 (B7RP-1) pathway. PMID: 19155489
45. These findings implicate ICOSL-induced IL-10, but not IDO in the regulation of BM-derived pDC function. PMID: 19208362
46. compared T cell responses of WT, CD28 knockout (KO), B7-H2 KO, and CD28-B7-H2 double KO (DKO) mice in a model of delayed-type hypersensitivity PMID: 19249753
47. This molecule acts as a costimulatory counterreceptor by augmenting the recall responses of CD8+ T cells. PMID: 11418641
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, BTN/MOG family
組織特異性：

Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the fo
數據庫鏈接：

KEGG: mmu:50723

STRING: 10090.ENSMUSP00000101032

UniGene: Mm.17819

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.