Mouse Fibrinogen Degradation Product,FDP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血纖蛋白原降解產(chǎn)物(FDP)酶聯(lián)免疫試劑盒（CSB-E07941m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FDP含量。FDP即果糖二磷酸，它在細(xì)胞能量代謝等方面有重要作用。其背景為機體糖代謝的中間產(chǎn)物。研究機制上，它能調(diào)節(jié)糖代謝中多種酶活性，增加細(xì)胞內(nèi)高能磷酸鍵和三磷酸腺苷含量，提高細(xì)胞對缺氧、缺血耐受性，利于細(xì)胞功能恢復(fù)。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，常用于心血管疾病、腫瘤轉(zhuǎn)移或炎癥反應(yīng)等機制研究，適用于評估小鼠模型中溶栓治療效果、血栓形成動態(tài)監(jiān)測或纖維蛋白代謝相關(guān)研究，為纖溶系統(tǒng)調(diào)控及抗凝藥物開發(fā)提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

FDP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-97
1:200	Average %	93
	Range %	88-98
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

回收率：

The recovery of mouse FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	92	88--95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.