Mouse Claudin-5(CLDN5) ELISA kit
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中文名稱：小鼠封閉蛋白5(CLDN5)酶聯(lián)免疫試劑盒
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貨號：CSB-EL005507MO
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠封閉蛋白5(CLDN5)酶聯(lián)免疫試劑盒（CSB-EL005507MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CLDN5含量。CLDN5即閉合蛋白5，是緊密連接蛋白家族成員，在血管內(nèi)皮細(xì)胞廣泛表達(dá)，對維持血腦屏障完整性至關(guān)重要。研究發(fā)現(xiàn)其可作為腫瘤治療靶點(diǎn)，通過調(diào)節(jié)其表達(dá)或功能，影響腫瘤血管生成和轉(zhuǎn)移，為腫瘤診斷與治療提供新思路。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，本試劑盒通過特異性捕獲抗體與檢測抗體的協(xié)同作用，可實(shí)現(xiàn)目標(biāo)蛋白的高效識別與定量分析，適用于生命科學(xué)研究中探索CLDN5在組織屏障功能調(diào)控、疾病模型建立、藥物干預(yù)效果評估等領(lǐng)域的表達(dá)變化規(guī)律。為研究者提供了一種穩(wěn)定、可靠的檢測工具，適用于體外實(shí)驗(yàn)?zāi)Ｐ椭蠧LDN5蛋白水平的動態(tài)監(jiān)測及相關(guān)分子機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Cldn5; Bec1; Claudin-5; Brain endothelial cell clone 1 protein; Lung-specific membrane protein
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縮寫：CLDN5
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：18.75 pg/mL-1200 pg/mL
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靈敏度：4.68 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 101 Range % 95-104 1:2 Average % 95 Range % 90-99 1:4 Average % 92 Range % 87-98 1:8 Average % 92 Range % 86-97 -
回收率：
The recovery of mouse CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 83-92 EDTA plasma (n=4) 93 87-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1200 2.312 2.422 2.367 2.240 600 1.846 1.966 1.906 1.779 300 1.261 1.229 1.245 1.118 150 0.754 0.769 0.762 0.635 75 0.443 0.456 0.450 0.323 37.5 0.271 0.281 0.276 0.149 18.75 0.208 0.202 0.205 0.078 0 0.126 0.128 0.127 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Gut microbiota-derived melatonin from Puerariae Lobatae Radix-resistant starch supplementation attenuates ischemic stroke injury via a positive microbial co-occurrence pattern Z Lian,Pharmacological research,2023
- Gliovascular disruption and cognitive deficits in a mouse model with features of small vessel disease Holland PR et al,J Cereb Blood Flow Metab,2015
產(chǎn)品評價
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靶點(diǎn)詳情
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功能：Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
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基因功能參考文獻(xiàn)：
- This study demonstrated that the claudin-5(+) leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles. PMID: 27852330
- Claudin 5 in a murine model of allergic asthma: Its implication and response to steroid treatment PMID: 26409663
- while MMP-9 is not essential for hypoxic-induced cerebral angiogenesis, it plays an important role in post-hypoxic vascular pruning by degrading laminin and claudin-5 PMID: 25812799
- Studied if circulating TGFbeta1 drives changes in tight junction protein expression and MMP9 activity following acute liver failure; Mouse brain endothelial cells were treated with recombinant rTGFbeta1 and MMP9 and claudin-5 expression was assessed. PMID: 26006017
- In transgenic mice carrying mutated SOD1 genes, a disrupted blood-spinal cord barrier as well as decreased levels of tight junction proteins ZO-1, occludin, and claudin-5 were detected. PMID: 25853911
- The aim of the present study was to further characterize the estrogen-responsive elements of claudin-5 promoter. PMID: 24846172
- Two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. PMID: 25281324
- Comparative modelling of intramolecular interfaces in the transmembrane region of claudins led to a complete Cldn5 model. PMID: 25174580
- silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293
- Claudin 5 showed identical mRNA expression levels in the brains of adult and aged mice. PMID: 22120415
- TNFalpha acts through NFkappaB signaling and requires a conserved promoter region for the down-regulation of Cldn5 expression. PMID: 22138107
- In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956
- These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
- This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806
- envision that substances interfering with Cldn5-mediated paracellular barrier tightening could assist an improved drug delivery into the brain because Cldn5 is essential for blood-brain barrier integrity. PMID: 20080584
- Describe claudin-5 as a novel estrogen target in vascular endothelium and show in vivo (brain endothelium) and in vitro (brain and heart endothelium) effects of estrogen on claudin-5 levels. PMID: 19910637
- tight junctions in dermal vascular endothelial cells are composed of claudin-5. PMID: 12823443
- claudin-5 is specifically altered in utrophin/dystrophin-deficient (double knockout, dko hearts, suggesting that alterations of the lateral membranes of cardiomyocytes PMID: 15698839
- Measured changes in claudin5 after cardiac arrest in mouse lung in relation to warm ischemia time and lung injury. PMID: 18281348
- Amyotrophic lateral sclerosis-linked superoxide dismutase 1 (SOD1) mutants with different biochemical characteristics disrupted the blood-spinal cord barrier in mice by reducing the levels of the tight junction protein claudin-5. PMID: 18344992
- Hydrodynamic tail vein injection of Cldn5 siRNA attenuates Cldn5 expression. PMID: 18509865
- Tissue-specific regulation of the claudin-5 gene via glucocorticoids and a high vulnerability of claudin-5 to TNF alpha. PMID: 18996436
- the pro-inflammatory chemokine CCL2 mediates brain endothelial barrier disruption during CNS inflammation PMID: 19423710
- CLDN5 is expressed in the seminiferous epithelium, appears to be regulated by multiple influences, and contributes to blood-testis barrier function. PMID: 19571261
- Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure. PMID: 19821483
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亞細(xì)胞定位：Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
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蛋白家族：Claudin family
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組織特異性：Widely expressed with highest levels in the lung.
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數(shù)據(jù)庫鏈接：
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