Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit
-
中文名稱：小鼠心臟營養(yǎng)素樣細胞因子1(CLCF1)酶聯(lián)免疫試劑盒
-
貨號：CSB-EL005479MO
-
規(guī)格：96T/48T
-
價格：￥3600/￥2500
-
其他：
產品詳情
-
產品描述：小鼠心臟營養(yǎng)素樣細胞因子1(CLCF1)酶聯(lián)免疫試劑盒（CSB-EL005479MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CLCF1含量。CLCF1 即睫狀神經營養(yǎng)因子樣細胞因子 1，可參與多種生理病理過程。在研究機制方面，它能與細胞表面受體結合，激活下游信號通路，影響細胞生長、分化、存活等。對其深入研究有望為相關疾病如神經系統(tǒng)疾病、癌癥等治療提供新靶點。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，該產品可廣泛應用于基礎科研領域，如心血管疾病動物模型中CLCF1的分泌動態(tài)研究、體外培養(yǎng)細胞因子釋放機制分析，以及心臟組織損傷修復相關的分子機制探索。實驗操作流程標準化，兼容常規(guī)酶標儀檢測，為研究者提供穩(wěn)定高效的CLCF1定量分析工具，滿足神經科學、心血管研究及免疫調節(jié)等方向的實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：Clcf1 ELISA kit; Bsf3 ELISA kit; Nnt1Cardiotrophin-like cytokine factor 1 ELISA kit; B-cell-stimulating factor 3 ELISA kit; BSF-3 ELISA kit; Novel neurotrophin-1 ELISA kit; NNT-1 ELISA kit
-
縮寫：CLCF1
-
Uniprot No.：
-
種屬：Mus musculus (Mouse)
-
樣本類型：serum, plasma, cell culture supernates, tissue homogenates
-
檢測范圍：23.5 pg/mL-1500 pg/mL
-
靈敏度：5.8 pg/mL
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Others
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-94 1:2 Average % 89 Range % 85-93 1:4 Average % 103 Range % 98-109 1:8 Average % 85 Range % 80-90 -
回收率：
The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-100 EDTA plasma (n=4) 91 85-97 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1500 2.401 2.293 2.347 2.204 750 1.927 1.876 1.902 1.759 375 1.315 1.361 1.338 1.195 187.5 0.869 0.837 0.853 0.710 94 0.574 0.584 0.579 0.436 47 0.338 0.319 0.329 0.186 23.5 0.232 0.245 0.239 0.096 0 0.146 0.140 0.143 -
數據處理：
-
貨期：3-5 working days
產品評價
相關產品
靶點詳情
-
功能：In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
-
基因功能參考文獻：
- Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
- CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain PMID: 12090754
- IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review] PMID: 15253382
- In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively. PMID: 19098279
-
亞細胞定位：Secreted.
-
蛋白家族：IL-6 superfamily
-
數據庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
