Mouse C-type lectin domain family 18 member A(CLEC18A) ELISA kit

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中文名稱：

小鼠C型凝集素結(jié)構(gòu)域家族18成員A(CLEC18A)酶聯(lián)免疫試劑盒
貨號：

CSB-EL005521MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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小鼠C型凝集素結(jié)構(gòu)域家族18成員A(CLEC18A)酶聯(lián)免疫試劑盒（CSB-EL005521MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CLEC18A含量。CLEC18A是一種C型凝集素，廣泛分布于人體各個器官，尤其在肝臟和骨髓細(xì)胞中含量最高。其研究機(jī)制主要涉及其在抗病毒免疫反應(yīng)中的作用，與TLR3結(jié)合增強(qiáng)免疫信號傳遞，并通過基因變異影響免疫反應(yīng)。此外，CLEC18A在肝臟纖維化和脂肪肝的病理過程中也扮演重要角色。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于探索CLEC18A在感染性疾病、自身免疫模型或炎癥機(jī)制研究中的動態(tài)表達(dá)規(guī)律，亦可應(yīng)用于基因編輯動物模型中蛋白表達(dá)水平的驗(yàn)證，為免疫調(diào)控機(jī)制及分子信號通路研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Clec18a ELISA kit; Mrcl ELISA kit; MrlpC-type lectin domain family 18 member A ELISA kit; Mannose receptor-like protein ELISA kit
縮寫：

CLEC18A
Uniprot No.：

Q7TSQ1
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC18A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	94-103

回收率：

The recovery of mouse CLEC18A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	102	98-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.546	2.500	2.523	2.402
10	1.947	2.010	1.979	1.858
5	1.419	1.368	1.394	1.273
2.5	0.831	0.842	0.837	0.716
1.25	0.525	0.536	0.531	0.410
0.625	0.357	0.371	0.364	0.243
0.312	0.267	0.251	0.259	0.138
0	0.122	0.120	0.121

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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基因功能參考文獻(xiàn)：
1. Dendritic cells use the mannose receptor for endocytosis of a particular antigen type (soluble ovalbumin) intended for cross-presentation. PMID: 16709836
亞細(xì)胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

UniGene: Mm.135495

Mouse C-type lectin domain family 18 member A(CLEC18A) ELISA kit

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