Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit

Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit

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文獻(xiàn)引用 (6) 評(píng)價(jià) (2) 說(shuō)明書(shū)下載

中文名稱(chēng)：

小鼠抗環(huán)胍氨酸肽抗體(Anti-CCP-antibody)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EQ027743MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
引用文獻(xiàn)

產(chǎn)品描述：

小鼠抗環(huán)胍氨酸肽抗體(Anti-CCP-antibody)酶聯(lián)免疫試劑盒（CSB-EQ027743MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的Anti-CCP-Ab含量。Anti - CCP - Ab即抗環(huán)瓜氨酸肽抗體，它是類(lèi)風(fēng)濕關(guān)節(jié)炎高度特異的血清學(xué)標(biāo)志物。其研究機(jī)制主要是能識(shí)別環(huán)化修飾的抗原決定簇，與類(lèi)風(fēng)濕關(guān)節(jié)炎發(fā)病機(jī)制中的自身免疫反應(yīng)相關(guān)，檢測(cè)它有助于類(lèi)風(fēng)濕關(guān)節(jié)炎的早期診斷及病情評(píng)估。試劑盒檢測(cè)范圍為6.25 U/mL-400 U/mL，適用于基礎(chǔ)科研中疾病機(jī)制探索、藥物研發(fā)階段的藥效學(xué)評(píng)價(jià)以及動(dòng)物模型中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測(cè)等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

Anti-CCP-Ab
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma
檢測(cè)范圍：

6.25 U/mL-400 U/mL
靈敏度：

1.56 U/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-101
1:2	Average %	97
	Range %	91-106
1:4	Average %	95
	Range %	90-106
1:8	Average %	96
	Range %	89-104

回收率：

The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-110
EDTA plasma (n=4)	95	83-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
400	2.697	2.741	2.719	2.573
200	2.201	2.224	2.213	2.067
100	1.507	1.483	1.495	1.349
50	0.834	0.849	0.842	0.696
25	0.492	0.511	0.502	0.356
12.5	0.297	0.310	0.304	0.158
6.25	0.226	0.238	0.232	0.086
0	0.148	0.144	0.146

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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平均分:

5.0分 - 2 個(gè)評(píng)價(jià)

樣品類(lèi)型：Serum

樣品信息：小鼠

稀釋比：其他

產(chǎn)品評(píng)價(jià)：我用anti-CCP試劑盒檢測(cè)小鼠正常、模型、給藥組的血清樣本，數(shù)據(jù)比較穩(wěn)定，組內(nèi)差異小。個(gè)別樣本復(fù)測(cè)值差別也很小，試劑盒質(zhì)量穩(wěn)定。以前買(mǎi)的試劑盒剩余了幾個(gè)孔，大概過(guò)了半年多再用，還是很不錯(cuò)，贊！希望華美繼續(xù)保持！

By 呂老師

樣品類(lèi)型：Serum

樣品信息：小鼠

稀釋比：其他

產(chǎn)品評(píng)價(jià)：試劑盒質(zhì)量很好，測(cè)試很穩(wěn)定，標(biāo)曲也很好。

By 賓老師

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.