Mouse α-Melanocyte Stimulating Hormone(α-MSH) ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠α-黑色素細胞刺激素(α-MSH)酶聯免疫試劑盒（CSB-E15876m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的α-MSH含量。α - MSH即α - 促黑素細胞激素，它是一種內源性神經肽。背景上，其在體內多種生理調節中起重要作用。研究機制方面，它能與相應受體結合，參與調節黑素生成、能量代謝、免疫反應及炎癥調控等過程，是當前生物學和醫學領域的研究熱點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外實驗模型中α-MSH分泌水平的動態監測，例如評估藥物干預對神經內分泌通路的影響、炎癥模型中肽類激素變化分析，或黑色素細胞功能相關的基礎研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

alpha-melanocyte stimulating hormone, alpha-MSH
縮寫：

α-MSH
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-95
1:2	Average %	92
	Range %	88-99
1:4	Average %	94
	Range %	90-99
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of mouse α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	95	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.