Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

產品詳情

文獻引用 (5) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人α-黑色素細胞刺激素(α-MSH)酶聯免疫試劑盒（CSB-E09658h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的α-MSH含量。α - MSH即α - 促黑素細胞激素，是一種內源性神經肽。它在調節皮膚色素沉著、能量代謝、炎癥反應等方面發揮重要作用。研究機制主要涉及與黑素皮質素受體結合，激活下游信號通路，進而對多種生理功能產生影響。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產品廣泛應用于基礎科研領域，如探究α-MSH在代謝綜合征動物模型中的表達特征、評估體外細胞刺激實驗的激素分泌水平，或解析皮膚黑色素生成調控機制等研究場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

alpha-melanocyte stimulating hormone, alpha-MSH
縮寫：

α-MSH
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	95
	Range %	91-104
1:4	Average %	89
	Range %	85-93
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	94	89-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個評價

樣品類型：細胞培養上清液

樣品信息：人

稀釋比：沒有稀釋

產品評價：我用的是人源α-MSH試劑盒。本次實驗目的：探究UVB對角質細胞分泌α-MSH的影響及多肽樣品的作用。實驗樣品：UVB照射后，細胞培養箱中孵育24h，收取細胞分泌上清液。實驗組OD值是0.15-0.31。以上為近期所用后的反饋，請查收！

By 雷老師