Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

產品詳情

文獻引用 (13) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Ⅰ型前膠原N端前肽(PⅠNP)酶聯免疫試劑盒（CSB-E11226h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅠNP含量。PⅠNP即I型前膠原氨基端前肽，它是反映成骨細胞活性和骨形成的特異性指標。其背景在于可用于評估骨代謝狀態。研究機制上，主要是通過檢測血液中PⅠNP水平，來反映骨合成代謝過程，輔助診斷和監測骨質疏松癥等骨病。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于骨組織工程研究、骨骼疾病動物模型評估、骨代謝相關分子機制解析等科研場景，為骨代謝研究、抗骨質疏松藥物篩選及骨再生材料開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
縮寫：

PⅠNP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	89-102
1:200	Average %	90
	Range %	83-97
1:400	Average %	99
	Range %	94-105
1:800	Average %	96
	Range %	90-102

回收率：

The recovery of human PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	101	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.