Human pro-gastrin-releasing peptide, ProGRP ELISA Kit

Human pro-gastrin-releasing peptide, ProGRP ELISA Kit

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文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

中文名稱(chēng)：

人胃泌素釋放肽前體(ProGRP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09127h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

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人胃泌素釋放肽前體(ProGRP)酶聯(lián)免疫試劑盒（CSB-E09127h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ProGRP含量。ProGRP（胃泌素釋放肽前體）是一種在小細(xì)胞肺癌（SCLC）中特異性高表達(dá)的腫瘤標(biāo)志物。它作為SCLC發(fā)生發(fā)展中的生物標(biāo)志物，可能具有致癌基因的作用，并可能成為潛在的治療靶點(diǎn)。研究顯示，ProGRP在SCLC的診斷、療效評(píng)估和預(yù)后預(yù)測(cè)中具有重要作用。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，適用于腫瘤標(biāo)志物篩選、疾病機(jī)制研究及抗腫瘤藥物開(kāi)發(fā)等科研領(lǐng)域，尤其為肺癌相關(guān)生物標(biāo)志物探索、病理模型驗(yàn)證及組織微環(huán)境分析提供可靠工具，其優(yōu)化的檢測(cè)體系支持多樣本類(lèi)型的高通量檢測(cè)需求，滿足實(shí)驗(yàn)室基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)應(yīng)用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

ProGRP
種屬：

Homo sapiens (Human)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	80-90
1:10	Average %	97
	Range %	92-101
1:20	Average %	98
	Range %	93-102
1:40	Average %	93
	Range %	86-96

回收率：

The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	97	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.432	2.327	2.380	2.253
600	1.894	1.886	1.890	1.763
300	1.271	1.237	1.254	1.127
150	0.721	0.692	0.707	0.580
75	0.467	0.452	0.460	0.333
37.5	0.338	0.345	0.342	0.215
18.75	0.238	0.225	0.232	0.105
0	0.129	0.125	0.127

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

High Sensitive Immunoelectrochemical Measurement of Lung Cancer Tumor Marker ProGRP Based on TiO2-Au Nanocomposite Zheng Wei, et al,Molecules,2019
The prognostic value of the serum neuron specific enolase and lactate dehydrogenase in small cell lung cancer patients receiving first-line platinum-based Liu X.et al,Medicine (Baltimore),2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.