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中文名稱：人血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒
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貨號：CSB-E14339h
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒（CSB-E14339h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的KLKB1含量。KLKB1，即血漿激肽釋放酶（KLKB1），是一種參與血液凝固、纖維蛋白溶解、激肽生成和炎癥的表面依賴激活的糖蛋白。其研究機制涉及基因突變、多態性與疾病如肺血栓栓塞癥的相關性，以及通過CRISPR-Cas9基因編輯技術治療遺傳性血管性水腫等。試劑盒檢測范圍為9.38 mU/mL-600 mU/mL，該產品特別適用于基礎科研領域，可用于研究KLKB1在心血管疾病、炎癥反應、纖維化疾病中的功能調控機制，或用于評估藥物干預對激肽釋放酶系統的影響，為探索相關疾病的分子機制及潛在治療靶點提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Fletcher factor ELISA Kit; kallikrein B plasma ELISA Kit; Kallikrein B plasma (Fletcher factor) 1 ELISA Kit; Kallikrein B; plasma 1 ELISA Kit; Kallikrein B1 ELISA Kit; Kininogenin ELISA Kit; KLK3 ELISA Kit; KLKB1 ELISA Kit; KLKB1_HUMAN ELISA Kit; PKK ELISA Kit; PKKD ELISA Kit; Plasma kallikrein ELISA Kit; Plasma kallikrein B1 ELISA Kit; Plasma kallikrein light chain ELISA Kit; Plasma prekallikrein ELISA Kit; PPK ELISA Kit; Prekallikrein ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma
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檢測范圍：9.38 mU/mL-600 mU/mL
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靈敏度：2.34 mU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 89-100 1:200 Average % 90 Range % 85-98 1:400 Average % 87 Range % 83-93 1:800 Average % 92 Range % 88-96 -
回收率：
The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 80-103 EDTA plasma (n=4) 96 86-106 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 600 2.101 2.207 2.154 2.048 300 1.695 1.687 1.691 1.585 150 1.261 1.278 1.270 1.164 75 0.842 0.867 0.855 0.749 37.5 0.503 0.514 0.509 0.403 18.75 0.345 0.368 0.357 0.251 9.38 0.201 0.211 0.206 0.100 0 0.105 0.107 0.106 -
數據處理：
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貨期：3-5 working days
引用文獻
- Proteomic Analysis of Serum from Patients with Major Depressive Disorder to Compare Their Depressive and Remission Statuses Jiyeong Lee et al, Psychiatry Investig,2015
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靶點詳情
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功能：The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
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基因功能參考文獻：
- We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development. PMID: 29186361
- Plasma kallikrein (PLK)-dependent transforming growth factor-beta (TGF-beta) activation could be detected in isolated pancreatic stellate cells (PSCs) from chronic pancreatitis (CP) and pancreatic cancer. PMID: 28187111
- High molecular weight kinin functions as a cofactor for PK activation in the presence of nucleic acids in a manner consistent with classic models of contact activation. PMID: 28124063
- genetic polymorphism is associated with C1 Inhibitor deficiency angioedema age of onset in European families PMID: 29130992
- these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1 PMID: 26566265
- von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth. PMID: 27581227
- KLKB1 mRNA expression is a putative molecular biomarker in chronic lymphocytic leukemia. PMID: 25891023
- Genotyping these subjects revealed that the carriers of the minor alleles at the two loci- F12 and KLKB1 had a significant association with reduced levels of active plasma renin. PMID: 26969407
- 2 genetic loci (kininogen 1 and kallikrein B) influencing key components of the RAAS, consistent with the close interrelation between the kallikrein-kinin system and the RAAS. PMID: 25477429
- PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
- Prekallikrein deficiency in a family is due to a new mutation (Arg541Gln) in exon 14. PMID: 25075649
- FXI may have a role in risk of ischemic stroke, but not myocardial infarct; FXII and prekallikrein may not have a role in either PMID: 24977287
- The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II. PMID: 23672780
- Investigate the relationship between circulating PK levels in children with metabolic syndrome and CVD risk factors because PK may be involved in the progression of the disease state. PMID: 20725119
- Case Report: Fresh frozen plasma transfusion before coronary artery bypass corrects prekallikrein deficiency. PMID: 20135073
- plasma kallikrein and FXIa activate pro-HGF in vitro PMID: 12372819
- the effect of kininogen degradation by human neutrophil elastase (HNE) on kinin generation by tissue and plasma kallikreins. PMID: 12887060
- prekallikrein preferential binding to endothelial cells contributes to their anticoagulant nature PMID: 12944405
- Crystallization and x-ray crystal structure determination have yielded the first three-dimensional views of the catalytic domain of plasma kallikrein PMID: 16199530
- Our findings suggested that common genetic variation in the KLKB1 gene might contribute to the risk of hypertension in the northern Han Chinese population. PMID: 17318641
- study demonstrates by immunohistochemistry that plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues PMID: 17696780
- three-dimensional structural data for prekallikrein (PK); interaction between PK and high-molecular-weight kininogen is mediated by two discrete surfaces formed by the PK A1, A2 and A4 domains with charge likely to be a critical component of the binding PMID: 17922805
- Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway PMID: 18160576
- Kallikrein is responsible for the cleavage of factor H. PMID: 18413232
- prekallikrein is an enzyme that can cleave high-molecular-weight kininogen to release bradykinin, and this reaction is inhibited by C1 inhibitor. PMID: 19342086
- NPY3-35 is a new peptide generated by plasma kallikrein PMID: 19620246
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相關疾病：Prekallikrein deficiency (PKK deficiency)
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亞細胞定位：Secreted.
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蛋白家族：Peptidase S1 family, Plasma kallikrein subfamily
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數據庫鏈接：
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